Caudal-type homeobox 2 (CDX2) transcription factor is an important marker for early trophoblast lineages and intestinal epithelium. Due to its nuclear expression the immunostaining and sorting of viable CDX2 cells is not possible. In this paper we report the generation and describe key characteristics of a CDX2 knock-in reporter hiPSC-cell line (MHHi007-A-1) which can serve as an in vitro tool to study human trophoblast and intestinal differentiation.
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View Article and Find Full Text PDFGeneration of mesenchyme free intestinal organoids from human induced pluripotent stem cells.
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Article Synopsis
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Centre for Cellular and Molecular Biology, Hyderabad, India. Electronic address:
Caudal-type homeobox 2 (.Cdx2) transcription factor is an essential regulator of differentiation to the intestinal epithelium, somatic mesoderm and trophectoderm function in the mouse. However, the regulation of Cdx2 in these processes is poorly understood.
View Article and Find Full Text PDFGeneration of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation.
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Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany. Electronic address:
Caudal-type homeobox 2 (CDX2) transcription factor is an important marker for early trophoblast lineages and intestinal epithelium. Due to its nuclear expression the immunostaining and sorting of viable CDX2 cells is not possible. In this paper we report the generation and describe key characteristics of a CDX2 knock-in reporter hiPSC-cell line (MHHi007-A-1) which can serve as an in vitro tool to study human trophoblast and intestinal differentiation.
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