High throughput DNA sequencing methodology (next generation sequencing; NGS) has rapidly evolved over the past 15 years and new methods are continually being commercialized. As the technology develops, so do increases in the number of corresponding applications for basic and applied science. The purpose of this review is to provide a compendium of NGS methodologies and associated applications. Each brief discussion is followed by web links to the manufacturer and/or web-based visualizations. Keyword searches, such as with Google, may also provide helpful internet links and information. © 2018 by John Wiley & Sons, Inc.
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http://dx.doi.org/10.1002/cpmb.59 | DOI Listing |
Small Methods
January 2025
School of Pharmacy, Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih, Selangor Darul Ehsan, 43500, Malaysia.
Hybridization chain reaction (HCR) and DNA-templated silver nanoclusters (AgNCs) have emerged as powerful tools in biosensing. HCR enables cascade amplification through programmable DNA interactions, while DNA-AgNCs serve as transducing units with unique fluorogenic and electrochemical properties. Integrating these components into a hybrid sensor could significantly enhance sensing capabilities across various fields.
View Article and Find Full Text PDFACS Sens
January 2025
Chimie des Interactions Plasma Surface group, Chemistry Department, Université de Mons, 7000 Mons, Belgium.
Borophene, a two-dimensional allotrope of boron, has emerged as a promising material for gas sensing because of its exceptional electronic properties and high surface reactivity. This review comprehensively overviews borophene synthesis methods, properties, and sensing applications. However, it is crucial to acknowledge the substantial gap between the abundance of theoretical literature and the limited experimental studies.
View Article and Find Full Text PDFNanoscale
January 2025
Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, USA.
Additive manufacturing (AM) of magnetic materials has recently attracted increasing interest for various applications but is often limited by the high cost and supply chain risks of rare-earth-element (REE) magnetic precursors. Recent advances in nanomanufacturing have enabled the development of rare-earth-free (REF) magnetic materials, such as spinel ferrites, hexaferrites, MnAl, MnBi, Alnico, FePt, and iron oxides/nitrides, which offer promising alternatives for printing high-performance magnetic devices. This review provides a detailed overview of the latest developments in REF magnetic materials, covering both synthesis strategies of REF magnetic materials/nanomaterials and their integration into AM processes.
View Article and Find Full Text PDFFront Plant Sci
December 2024
Nuclear Institute for Agriculture and Biology College, Pakistan Institute of Engineering and Applied Science (NIAB-C, PIEAS), Faisalabad, Pakistan.
Accessing the underlying genetics of complex traits, especially in small grain pulses is an important breeding objective for crop improvement. Genome-wide association studies (GWAS) analyze thousands of genetic variants across several genomes to identify links with specific traits. This approach has discovered many strong associations between genes and traits, and the number of associated variants is expected to continue to increase as GWAS sample sizes increase.
View Article and Find Full Text PDFAn Pediatr (Engl Ed)
December 2024
Grupo de investigación en enfermedades raras, Laboratorio de (epi)genética molecular, Instituto de Investigación Sanitaria Bioaraba, Hospital Universitario Araba, Vitoria-Gasteiz, Spain. Electronic address:
Advances in next-generation sequencing (NGS) technologies have made the detection of the molecular causes of paediatric diseases increasingly affordable, accessible and rapid. While exome sequencing and genome sequencing were until recently only available for research, they are now used in health care practice. The clinical application of NGS has raised many challenges in genetic counselling for families in terms of the interpretation of test results and incidental findings, as well as technical limitations in the event of inconclusive results.
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