AI Article Synopsis
- The study investigates the role of ETS proto-oncogene 1 (ETS-1) in glioblastoma, focusing on its expression in tumor cells and the potential influence of hypoxia.
- Immunohistochemical analysis showed that ETS-1 is present in stromal and endothelial cells of glioblastoma, with its levels relating to the density of blood vessels in the tumor.
- However, the research concluded that the expression of ETS-1 in glioblastoma cells is not regulated by hypoxia, indicating it's more associated with tumor vascularization.
Article Abstract
Background: Tumor cells infiltrating the brain are a typical hallmark of glioblastoma. Invasiveness of glioma cells has been associated with ETS proto-oncogene 1 (ETS-1). In non-glial tumors, ETS-1 expression has been linked to hypoxia. However, it is not known whether hypoxia regulates ETS-1 expression in glioblastoma.
Materials And Methods: The spatial distribution of ETS-1 expression in primary glioblastoma was assessed using immunohistochemistry. ETS-1 expression in glioblastoma-derived mesenchymal stem-like cells (gbMSLCs) was determined using immunocytochemistry. The effect of hypoxia on ETS-1 expression of gbMSLCs, glioma cell lines and glioblastoma-derived endothelial cells was assessed using polymerase chain reaction and immunoblotting.
Results: Our immunohistochemical studies revealed ETS-1 expression in stromal and endothelial glioblastoma cells. Stromal ETS-1 expression in glioblastoma correlated with microvessel density. gbMSLCs were found to express ETS-1. In all examined cell lines, ETS-1 transcription and expression were independent of hypoxia.
Conclusion: In glioblastoma, ETS-1-expression is not dependent on hypoxia, but correlates with tumor vascularization.
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| http://dx.doi.org/10.21873/anticanres.12601 | DOI Listing |
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