AI Article Synopsis
- The study examines the effects of methanol leaf extract (MeOHL) on calpain activity, melanin production, and DNA mutagenicity in colorectal cells.
- Chemical analysis of the extract reveals the presence of para-hydroxybenzoic acid, glycosylated kampferol, and 19 amino acids, suggesting a complex composition.
- Results demonstrate that MeOHL shows high cytotoxicity (up to 89.23%) and antioxidant effects, significantly boosts melanin production, and inhibits mutagenicity by 76.32% and 91% in different assays.
Article Abstract
In this study, we investigate the potential of methanol leaf extract (MeOHL) on calpain activity, melanin biosynthesis and DNA mutagenicity. Cytotoxic effect and measurement of reactive oxygen species (ROS) induced by lucigenin in colorectal cells (BE) were also determined. In addition, the chemical analysis of the extract was also studied and the chemical profile illustrates its content in para-hydroxybenzoic acid (-HBA), a glycosylated kampferol (GK), a glycosylated kampferol with Rhamnose (GKR) and 19 amino acids (AAs). Our results showed that MeOHL extract enhanced a significant cytotoxic (max of IP = 89.23%) and antioxidant (max of IP=100%) activities. Furthermore, the tested extract stimulated calpain activity and significantly increased the production of intra (46 µg/mL cells) and extracellular melanin content (12.5µg/mL). Using Ames assay, the extract exhibits a significant inhibition of mutagenicity induced by methy-methane-sulfonate (MMS) (76.32%) as well as 2-aminoanthracene (2-AA) (91%) in the TA104 assay system.
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