AI Article Synopsis
- Biologically active cyclotides, found in certain flowering plants, play a role in plant protection.
- Research focused on Brazilian plant species led to the detection of several cyclotides from the leaves and stems of the Violaceae family using LC-MS technology.
- A cyclotide named Nor A was isolated from stems, and its amino acid sequence was determined through MALDI-TOF-MS alongside various enzymatic digestion methods.
Article Abstract
Biologically active cyclotides have been found on some flowering plants species and are involved in the role of the plant protection. As part of studies focusing on peptides from Brazilian plant species, we are reporting the detection by LC-MS of several cyclotides from leaves and stems of (Violaceae). From stems it was possible to isolate and characterize a cyclotide named Nor A. Its primary structure (amino acid sequence) was established by MALDI-TOF-MS, based on the and type ion series, after reduction and alkylation reactions, as well as enzymatic digestion using the enzymes endoproteinase glutamic acid (endoGlu-C), trypsin, and chymotrypsin. Furthermore, the amino acid analysis was also described.
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