Abnormal regulation of long non-coding RNAs (lncRNAs) appears to be a primary feature of numerous types of human cancer. However, the association between the dysregulation of lncRNAs and functional alterations in gastric cancer (GC) remains unclear. In previous studies, we applied microarray and bioinformatics analyses to screen for key lncRNAs from the tumor tissues and matched adjacent non-tumor tissues of 10 patients with GC. There were seven key lncRNAs demonstrated to be significantly different between carcinoma tissues and adjacent non-tumor tissues. In the present study, the expression of these seven selected lncRNAs were validated in 82 patients with GC to further investigate the association between lncRNAs and GC clinical characterization. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) results demonstrated that , MCPH1 antisense RNA 1 () and urothelial carcinoma-associated 1 () exhibited consistent upregulation in cancer compared with adjacent non-tumor tissues, whereas , tumor suppressor candidate 8 () and maternally expressed 3 () showed consistent downregulation. The results from the microarray and RT-qPCR experiments achieved 100% agreement. A correlation analysis indicated that and were significantly associated with tumor differentiation degree, and were significantly associated with lymph node metastasis, and and were significantly associated with tumor-node-metastasis stage (P<0.05). In addition, it was identified that the differential expression of and significantly correlated with the age and gender of the GC patients, respectively (P<0.05). The results suggest that the lncRNAs and are closely associated with the invasion and metastasis of GC, which reveals these indicators as potential specificity biomarkers for the diagnosis, prognosis and classification of GC. Thus, these lncRNAs merit further study as novel candidate biomarkers for the clinical diagnosis of GC and as potential targets for therapy.
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| http://dx.doi.org/10.3892/ol.2018.8369 | DOI Listing |
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