AI Article Synopsis

  • TP53 mutations lead to the production of altered p53 proteins in various cancers, which lose normal functions and gain new ones that help tumors grow.
  • The study utilized the fact that mutant p53 proteins do not bind to H2AX, allowing researchers to create a new method to reduce the harmful functions of these mutations using a circular RNA molecule called circ-Ccnb1.
  • Results showed that circ-Ccnb1 not only inhibited tumor growth and prolonged mouse survival, but also altered protein interactions in cells, leading to cell death in those with p53 mutations, suggesting potential new strategies for combating cancers with p53 mutations.

Article Abstract

TP53 mutations occur in many different types of cancers that produce mutant p53 proteins. The mutant p53 proteins have lost wild-type p53 activity and gained new functions that contribute to malignant tumor progression. Different p53 mutations create distinct profiles in loss of wild-type p53 activity and gain of functions. Targeting the consequences generated by the great number of p53 mutations would be extremely complex. Therefore, in this study we used a workaround and took advantage of the fact that mutant p53 cannot bind H2AX. Using this, we developed a new approach to repress the acquisition of mutant p53 functions. We show here that the delivery of a circular RNA circ-Ccnb1 inhibited the function of three p53 mutations. By microarray analysis and real-time PCR, we detected decreased circ-Ccnb1 expression levels in patients bearing breast carcinoma. Ectopic delivery of circ-Ccnb1 inhibited tumor growth and extended mouse viability. Using proteomics, we found that circ-Ccnb1 precipitated p53 in p53 wild-type cells, but instead precipitated Bclaf1 in p53 mutant cells. Further experiments showed that H2AX serves as a bridge, linking the interaction of circ-Ccnb1 and wild-type p53, thus allowing Bclaf1 to bind Bcl2 resulting in cell survival. In the p53 mutant cells, circ-Ccnb1 formed a complex with H2AX and Bclaf1, resulting in the induction of cell death. We found that this occurred in three p53 mutations. These results shed light on the possible development of new approaches to inhibit the malignancy of p53 mutations.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261950PMC
http://dx.doi.org/10.1038/s41418-018-0115-6DOI Listing

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