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Analysis of the concentrations and size distributions of cell-free DNA in schizophrenia using fluorescence correlation spectroscopy. | LitMetric

Analysis of the concentrations and size distributions of cell-free DNA in schizophrenia using fluorescence correlation spectroscopy.

Transl Psychiatry

Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University, Shanghai, 200030, China.

Published: May 2018

AI Article Synopsis

  • Cell-free DNA (cfDNA), released during cell death, may indicate increased apoptotic activity in schizophrenia, a possibility explored through a study comparing patients with schizophrenia, mood disorders, and healthy controls.
  • The study found that cfDNA levels were approximately two times higher in schizophrenia patients than in healthy controls, with a significant difference that wasn’t observed in mood disorder patients.
  • Schizophrenia patients also exhibited shorter cfDNA molecules, suggesting a unique distribution pattern that could help develop cfDNA as a potential diagnostic marker for the disease.

Article Abstract

Cell-free DNA (cfDNA), which is primarily released following cell death, has been described and developed to serve as an effective biomarker in autoimmune diseases which may share the pathogenesis with schizophrenia. In this study, we hypothesized and explored whether the concentrations and size distributions of cfDNA are abnormal in schizophrenia. A total of 65 patients with schizophrenia (SZ), 29 patients with mood disorders (MD) and 62 matched healthy controls (HC) were included in the study. Fluorescence correlation spectroscopy was used to assay the molar concentrations and size distributions of cfDNA. Fluorometric quantification and quantitative real-time PCR (qPCR) were performed to verify the results. The cfDNA levels were approximately two-fold higher in the SZ group ((29 ± 15) nM) than in the healthy controls ((15 ± 9) nM; P-value = 0.00062), but the levels in patients with MD were not significantly different from those in the healthy controls ((17 ± 10) nM; P-value = 0.343). According to the size distribution analysis, cfDNA in schizophrenia patients was composed of shorter DNA molecules and showed an apoptosis-like distribution pattern. Our study shows the elevated levels and short sizes of cfDNA in schizophrenia patients, which provide direct evidences supporting increased apoptotic activity in the disease. cfDNA may be developed to serve as an auxiliary diagnostic marker for the disease in the future.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5966419PMC
http://dx.doi.org/10.1038/s41398-018-0153-3DOI Listing

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