Therapies based on regenerative techniques have the potential to radically improve healthcare in the coming years. As a result, there is an emerging need for non-destructive and label-free technologies to assess the quality of engineered tissues and cell-based products prior to their use in the clinic. In parallel, the emerging regenerative medicine industry that aims to produce stem cells and their progeny on a large scale will benefit from moving away from existing destructive biochemical assays towards data-driven automation and control at the industrial scale. Impedance-based cellular assays (IBCA) have emerged as an alternative approach to study stem-cell properties and cumulative studies, reviewed here, have shown their potential to monitor stem-cell renewal, differentiation and maturation. They offer a novel method to non-destructively assess and quality-control stem-cell cultures. In addition, when combined with disease models they provide complementary insights as label-free phenotypic assays. IBCA provide quantitative and very sensitive results that can easily be automated and up-scaled in multi-well format. When facing the emerging challenge of real-time monitoring of three-dimensional cell culture dielectric spectroscopy and electrical impedance tomography represent viable alternatives to two-dimensional impedance sensing.This article is part of the theme issue 'Designer human tissue: coming to a lab near you'.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974449 | PMC |
| http://dx.doi.org/10.1098/rstb.2017.0226 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Progressive Approaches in Oncological Diagnosis and Surveillance: Real-Time Impedance-Based Techniques and Advanced Algorithms.
Bioelectromagnetics
January 2025
Micropropulsion and Nanotechnology Laboratory, School of Engineering and Applied Science, George Washington University, Washington, DC, USA.
Cancer remains a formidable global health challenge, necessitating the development of innovative diagnostic techniques capable of early detection and differentiation of tumor/cancerous cells from their healthy counterparts. This review focuses on the confluence of advanced computational algorithms with noninvasive, label-free impedance-based biophysical methodologies-techniques that assess biological processes directly without the need for external markers or dyes. This review elucidates a diverse array of state-of-the-art impedance-based technologies, illuminating distinct electrical signatures inherent to cancer vs healthy tissues.
View Article and Find Full Text PDFExploring the association of dietary inflammatory index, disease progression and cellular integrity in chronic kidney disease: A cross-sectional study.
Nutr Health
November 2024
Department of Nephrology, Apollo BGS Hospitals, Mysuru, Karnataka, India.
Article Synopsis
- The study investigates the link between dietary inflammatory index (DII) scores and health markers in pre-dialysis chronic kidney disease (CKD) patients, revealing that overall diet quality influences kidney function and body composition.
- Early-stage CKD patients showed better body composition indicators, while higher DII scores were correlated with a decline in kidney function (eGFR) and cell integrity, particularly in late-stage CKD.
- Specific foods like cereals, millets, and certain vegetables were found to have anti-inflammatory benefits, highlighting the need for dietary considerations in CKD treatment.
TRPV4 Mediates Alveolar Epithelial Barrier Integrity and Induces ADAM10-Driven E-Cadherin Shedding.
Cells
October 2024
Walther Straub Institute for Pharmacology and Toxicology, Member of the German Center for Lung Research (DZL), Medical Faculty, LMU-Munich, Nussbaumstrasse 26, 80336 Munich, Germany.
Transient receptor potential vanilloid 4 (TRPV4) channels have been associated with numerous pulmonary pathologies, including hypertension, asthma, and acute lung injury. However, their role in the alveolar epithelium remains unclear. We performed impedance-based resistance measurements in primary differentiated alveolar epithelial type I (AT1) cells from wild-type (WT) and TRPV4-deficient (TRPV4-/-) C57/BL6J mice to detect changes in AT1 barrier integrity upon TRPV4 activation.
View Article and Find Full Text PDFCross-Scale Decoupling Kinetic Processes in Lithium-Ion Batteries Using the Multi-Dimensional Distribution of Relaxation Time.
Adv Sci (Weinh)
November 2024
Center for Ageing, Reliability and Lifetime Prediction of Electrochemical and Power Electronic Systems (CARL), Campus-Boulevard 89, 52074, Aachen, Germany.
Article Synopsis
- This study addresses how to non-destructively analyze the degradation mechanisms of lithium-ion batteries (LIBs) using a method called distribution of relaxation times (DRT).
- It introduces a new analytical framework to improve the resolution of timescales and reduce uncertainties by focusing on interfacial impedance and multi-dimensional DRT analysis.
- Results indicate that the new approach enhances detection accuracy of battery performance by approximately 20%, particularly at low state of charge and high temperatures, revealing critical differences in how various battery components impact performance.
Effect of Different Glucose Levels and Glycation on Meningioma Cell Migration and Invasion.
Int J Mol Sci
September 2024
Department of Neurosurgery, Medical Faculty, Martin-Luther-University Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle (Saale), Germany.
Article Synopsis
- - Meningiomas are mostly benign tumors but can have severe malignant forms, with research exploring the effects of glucose on their growth and behavior through diets low in glucose to potentially improve outcomes.
- - The study specifically analyzed how different glucose levels (low, normal, and high) affected a malignant meningioma cell line, examining migration and invasion using impedance-based methods and protein expression through immunoblotting.
- - Findings revealed that low glucose levels decreased the invasive capabilities of the malignant cells, while high glucose levels worsened their barrier function and adhesion, linked to lower expression of focal adhesion kinase (FAK).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!