AI Article Synopsis
- Actinic keratoses (AK) are skin growths caused by UV radiation that can progress to squamous cell carcinoma, especially in organ transplant recipients who are on immunosuppressive therapy.
- A clinical trial involving seven patients examined the effects of methyl aminolevulinate-photodynamic therapy (MAL-PDT) on AK, with skin biopsies taken before treatment and six weeks after.
- Results showed that MAL-PDT corrected abnormal gene expressions linked to cancer and promoted remodeling of damaged skin, confirming its effectiveness for treating AK and sun-damaged skin.
Article Abstract
Background: Actinic keratoses (AK) are proliferations of neoplastic keratinocytes in the epidermis resulting from cumulative exposure to ultraviolet radiation (UVR), which are liable to transform into squamous cell carcinoma (SCC). Organ Transplant Recipients (OTR) have an increased risk of developing SCC as a consequence of long-term immunosuppressive therapy. The aim of this study was to determine the molecular signature of AKs from OTR prior to treatment with methyl aminolevulinate-photodynamic therapy (MAL-PDT), and to assess what impact the treatment has on promoting remodeling of the photo-damaged skin.
Methods: Seven patients were enrolled on a clinical trial to assess the effect of MAL-PDT with biopsies taken at screening prior to the first treatment session (week 1), and six weeks after completion of final treatment (week 18). Whole-genome gene expression analysis was carried out on skin biopsies isolated from an AK lesion, an area surrounding the lesion, and a non-sun exposed region of the body. Quantitative PCR was utilized to confirm the differential expression of key genes.
Results: MAL-PDT treatment corrected abnormal proliferation-related gene profiles, corrected aberrantly expressed cancer-associated genes and induced expression of dermal extracellular matrix genes in photo-exposed skin.
Conclusion: The efficacy of the MAL-PDT on AK lesions was confirmed at whole-genome gene expression level. A transcriptional signature of remodeling, identified through assessing the effect of MAL-PDT on photodamaged skin, supports the use of MAL-PDT for treating photodamaged skin and field cancerized areas.
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| http://dx.doi.org/10.1016/j.jdermsci.2018.05.002 | DOI Listing |
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