AI Article Synopsis
- - Diabetic retinopathy (DR) leads to vision loss and is characterized by abnormal blood vessel growth in the retina, and this study explores how Asymmetric dimethylarginine (ADMA) affects this process.
- - Researchers induced diabetes in rats and observed increased ADMA levels in both the eyes of diabetic rats and retinal cells under low oxygen conditions, linked to enhanced cell growth and vascular formation.
- - The study concludes that elevated ADMA promotes retinal neovascularization in diabetes, with the process regulated by a protein called ephrinB2, suggesting potential targets for DR treatment.
Article Abstract
Diabetic retinopathy (DR) is a leading cause of vision loss with retinal neovascularization. This study aims to investigate whether Asymmetric dimethylarginine (ADMA) impacts the pathogenesis of DR via focusing on promoting retinal neovascularization and its underlying molecular mechanisms. Diabetic rats were induced by a single intraperitoneal injection of streptozotocin (STZ) for 20 weeks. ADMA levels in aqueous and the influence of hypoxia on ADMA and angiogenesis in RF/6A cells were examined. The effects and underlying molecular mechanisms of ADMA on neovascularization of RF/6A cells were further evaluated by administration of ADMA, DDAH siRNA or ephrinB2 siRNA. Results showed that ADMA levels were elevated in both aqueous from diabetic rats and culture medium in RF/6A cells pretreated with hypoxia. Administration of ADMA directly promoted proliferation, migration, adhesion and tube formation of RF/6A cells, which was further confirmed by DDAH1 siRNA or DDAH2 siRNA. In addition, ephrinB2 expression was increased under diabetic conditions, and the angiogenic effects of ADMA were blocked by ephrinB2 siRNA. In conclusion, ADMA contributes to the neovascularization of retina in diabetic mellitus, which is regulated by ephrinB2.
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| http://dx.doi.org/10.1016/j.vph.2018.05.004 | DOI Listing |
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