AI Article Synopsis
- A-Raf, part of the Raf kinase family, has unclear roles compared to its relatives B-Raf and C-Raf, prompting an investigation into its biological functions through proteomics.
- Researchers discovered 132 proteins that interact with A-Raf, confirming 12 of these interactions, indicating that A-Raf is involved in regulating apoptosis, RNA breakdown, GTPase activity, and cell adhesion.
- The study highlights the connection between A-Raf and all ten hallmarks of cancer, suggesting its involvement in cancer development and potentially other diseases and signaling pathways.
Article Abstract
A-Raf is a member of the Raf kinase family. Unlike B-Raf and C-Raf, the functions of A-Raf remain obscure. To gain more insight into the biological functions of A-Raf, we investigated the A-Raf interactome using proteomics. We found 132 proteins that interact with A-Raf and confirmed the interaction of 12 of these proteins with A-Raf by western blotting. Our data suggested that A-Raf regulates apoptosis, RNA catabolism, GTPase activity, and cell adhesion by interacting with proteins located in different cellular compartments. We identified all ten hallmarks of cancer in these interacting proteins, suggesting that A-Raf is involved in carcinogenesis. Our results also indicated that A-Raf may play a role in different diseases and signaling pathways. These findings have identified potential regulators of A-Raf and provide a systemic insight into its biological functions.
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| http://dx.doi.org/10.1016/j.bbapap.2018.05.009 | DOI Listing |
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