AI Article Synopsis
- Colorectal cancer (CRC) is a prevalent cancer that is influenced by various oncogenes, and this study focused on a gene called ITGBL1 to understand its role in CRC progression.
- The researchers analyzed a dataset containing CRC and normal colon tissues, identifying 318 differentially expressed genes (DEGs), with ITGBL1 being significantly up-regulated and linked to poorer survival outcomes for patients.
- The study conducted various experiments to assess the effects of ITGBL1 on cancer cell behaviors, revealing that it promotes cancer cell proliferation, migration, and invasion while suggesting it could be a valuable target for CRC treatment and a prognostic biomarker.
Article Abstract
Colorectal cancer (CRC) is one of the most common malignancies worldwide; its progression and prognosis are associated with oncogenes. The present study aimed to identify differentially expressed genes (DEGs) and explore the role and potential mechanism of integrin subunit β like 1 (ITGBL1) in CRC. The microarray dataset GSE41258 was used to screen DEGs involved in CRC. Survival analysis was performed to predict the prognosis of CRC patients. To validate ITGBL1 expression, immunohistochemistry, quantitative real-time PCR and western blotting were performed in CRC tissues and cells. Subsequently, the effects of ITGBL1 were evaluated through colony formation, cell proliferation, migration and invasion assays. Finally, we took advantage of Gene Ontology (GO) analysis and Gene Set Enrichment Analysis (GSEA) to explore potential function and mechanism of ITGBL1 in CRC. In our study, 182 primary CRC tissues and 54 normal colon tissues were contained in GSE41258 dataset. A total of 318 DEGs were screened, among which ITGBL1 was found to be significantly up-regulated in CRC, and its high expression was associated with shortened survival of CRC patients. Moreover, knockdown of ITGBL1 promoted CRC cell proliferation, migration and invasion. Finally, GO analysis revealed that ITGBL1 was associated with cell adhesion. GSEA indicated that ITGBL1 was enriched in ECM receptor interaction and focal adhesion. In conclusion, a novel oncogene ITGBL1 was identified and demonstrated to be associated with the progression and prognosis of CRC, which might be a potential therapeutic target and prognostic biomarker for CRC patients.
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| http://dx.doi.org/10.1016/j.biopha.2018.05.033 | DOI Listing |
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