[Effects of glycolytic pathway on generation of regulatory T cells during infection].

Objective: To assess the influence of glycolytic pathway on the proportion and numbers of regulatory T cells during infection.

Methods: A -infected mouse model was established, and C57/BL6 male mice infected with were subjected to intraperitoneal injections of with the glycolytic inhibitor 2-Deoxy-D-glucose (2DG) or PBS for 6 times, and then the cells from spleen or mesenteric lymph nodes (LNs) were isolated and analyzed by flow cytometry (FCM) to detect the percentage of Glut1CD4 T cells and Treg cells.

Results: The proportions of Glut1CD4 T cells in the spleen (43.58%±2.50% vs. 21.15%±0.96%; = 8.834, < 0.01) and mesenteric LNs (38.97%±1.97% vs. 28.40%±2.11%; = 3.662, < 0.05) were higher in the normal mice than those in the infected mice, and the percentages of Treg cells in the spleen (6.83%±0.21% vs. 13.30%±0.35%; = 15.65, < 0.01) and LNs (8.26%±0.15% vs. 14.37%±0.44%; = 13.14, < 0.01) were lower in the normal mice than those in the infected mice. In addition, the proportions of Treg cells in the spleen (15.50%±0.76% vs. 13.07%±0.15%; = 3.130, < 0.05) and LNs (17.00% ±0.41% vs. 13.83%±0.18%; = 6.947, < 0.01) were higher in the infected mice injected intraperitoneally with 2DG than those in the infected mice injected intraperitoneally with PBS.

Conclusions: Glycolytic pathway inhibits Treg differentiation in the spleen and mesenteric LNs of -infected mice.