AI Article Synopsis
- The way plants are cultivated and processed greatly affects the results of metabolomics experiments.
- The chapter outlines careful procedures for plant growth and sample extraction to prevent contamination and alterations in analyte composition.
- It focuses on two specific plant examples—tomato fruits and flax seeds—and discusses extraction methods for analyzing semipolar compounds and fatty acids using advanced techniques like LC-MS and GC-MS.
Article Abstract
The way plants are grown and samples are harvested, prepared, and extracted has a profound impact on the output of a metabolomics experiment. In this chapter, we detail the experimental procedures from plant cultivation to extract preparation, in order to avoid difficulties that could result in contamination or undesired changes of the analytes. Two plant organs are mentioned as examples: tomato fruits (Solanum lycopersicum) and flax seeds (Linum usitatissimum). Extractions designed for the untargeted analysis of semipolar compounds by liquid chromatography-mass spectrometry (LC-MS) and targeted analysis of fatty acids by gas chromatography-mass spectrometry (GC-MS) or gas chromatography with flame ionization detector (GC-FID) are described.
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| http://dx.doi.org/10.1007/978-1-4939-7819-9_8 | DOI Listing |
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