Regulatory effects of IgG-BF on hybridoma B cells. molecular characterization of variant cell lines.

Mol Immunol

Laboratoire d'Immunologie Cellulaire et Clinique, INSERM U.255, Paris, France.

Published: November 1988

Immunoglobulin G-binding factors (IgG-BF) produced by mouse T cells or hybridoma T cells (T2D4) were used to manipulate in vitro mouse hybridoma B cells. Both IgG production by, and proliferation of, these cells was inhibited by IgG-BF, or during co-cultures with IgG-BF-producing T2D4 cells. Thus, treatment of tumor B cells, besides its potential therapeutic use, represents an invaluable model for studying the regulation of Ig production by IgG-BF at a molecular level. To further analyze the molecular events induced by IgG-BF in B cell hybridomas, a set of variant clones of a hybridoma cell line (UN2) was isolated and variants were characterized for their Ig production and their Fc gamma R expression.

Download full-text PDF

Source
http://dx.doi.org/10.1016/0161-5890(88)90148-4DOI Listing

Publication Analysis

Top Keywords

hybridoma cells
12
cells
7
igg-bf
5
regulatory effects
4
effects igg-bf
4
hybridoma
4
igg-bf hybridoma
4
cells molecular
4
molecular characterization
4
characterization variant
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!