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A Multi-enzyme Protocol Improves Total Proteome Coverage in Extracellular Matrix.
Methods Mol Biol
December 2024
Department of Chemistry, North Carolina State University, Raleigh, NC, USA.
Extracellular matrix (ECM) from decellularized mammalian tissues has been used in many therapeutic applications. The tissue-specific composition of the ECM is critically associated with therapeutic performance. However, ECM translation needs to be improved because of the complex composition and limited understanding of ECM repairing mechanisms due partly to incomplete proteomic interrogation of ECM samples.
View Article and Find Full Text PDFDiagnostic value of laboratory markers of enteric dysfunction in preterm infants.
Wiad Lek
December 2024
BUKOVINIAN STATE MEDICAL UNIVERSITY, CHERNIVTSI, UKRAINE.
Objective: Aim: To study the peculiarities of food tolerance disorders in premature infants, taking into account the risk factors of gestational age and maternal labor, the peculiarities of the course of perinatal pathology, in order to determine pathogenetically sound clinical and laboratory criteria.
Patients And Methods: Materials and Methods: A comprehensive clinical and laboratory evaluation was performed on 67 preterm infants of gestational age 32 to 33/6 weeks with severe food tolerance disorders in perinatal pathology. The comparison group consisted of 31 newborns with gestational age of 34 to 37 weeks.
Association of leukocyte elastase in semen and seminal plasma with sperm parameters and pregnancy outcomes in male fertility.
Endocr Connect
December 2024
C Jayasena, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, SW7 2AZ, United Kingdom of Great Britain and Northern Ireland.
Background: Semen analysis is the standard test for evaluating male fertility. However, it may not address all aspects of male infertility. This review explores the role of leukocyte elastase (LE) as a possible biomarker for male fertility by evaluating 28 corresponding studies.
View Article and Find Full Text PDFEuropean guidelines for the diagnosis and treatment of pancreatic exocrine insufficiency: UEG, EPC, EDS, ESPEN, ESPGHAN, ESDO, and ESPCG evidence-based recommendations.
United European Gastroenterol J
December 2024
Department of Clinical Sciences, Karolinska Institutet and Department of Upper Abdominal Diseases, Karolinska University Hospital, Stockholm, Sweden.
Pancreatic exocrine insufficiency (PEI) is defined as a reduction in pancreatic exocrine secretion below the level that allows the normal digestion of nutrients. Pancreatic disease and surgery are the main causes of PEI. However, other conditions and upper gastrointestinal surgery can also affect the digestive function of the pancreas.
View Article and Find Full Text PDFAlpha-1-antitrypsin as novel substrate for Spl proteases - implications for virulence.
Front Immunol
December 2024
Institute of Clinical Chemistry and Laboratory Diagnostics, Jena University Hospital, Jena, Germany.
Background: The serine protease like (Spl) proteases of are a family of six proteases whose function and impact on virulence are poorly understood. Here we propose alpha-1-antitrypsin (AAT), an important immunomodulatory serine protease inhibitor as target of SplD, E and F. AAT is an acute phase protein, interacting with many proteases and crucial for prevention of excess tissue damage by neutrophil elastase during the innate immune response to infections.
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