Description of an in vivo oral mucosa HSV-1 infection model in mice.

Acta Odontol Latinoam

Universidad Nacional de Colombia, Facultad de Odontología, Grupo de Investigaciones Básicas y Aplicadas en Odontología, Bogotá D.C, Colombia.

Published: December 2017

In this work, we established an in vivo murine model of herpes simplex virus type 1 (HSV-1) infection involving inoculation by scarification of the oral mucosain order to study its dissemination towards the trigeminal ganglion (TG). Both viral DNA and infectious virions were detected on the third day postinfection (p.i.). Viral proteins revealed by immunohistochemistry were mainly found at seven days p.i., when the latency-associated transcript (LAT) was also detected. This model simulated the dissemination process of HSV-1, which could be used to study herpes pathogenesis starting in the oral mucosa.

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