AI Article Synopsis
- Phage O4 of Pseudomonas aeruginosa was identified as a short-tailed virus and its genome consists of a linear double-stranded DNA molecule with 50,509 base pairs and 76 predicted genes.
- Mass spectrometry analysis showed that the O4 virus contains various proteins, including structural proteins, enzymes, and hypothetical proteins.
- Eight specific genes related to lipopolysaccharide synthesis were confirmed to be responsible for phage resistance, marking O-specific antigen as the receptor for phage O4 absorption; the genetic makeup indicates O4 is a novel member of the Podoviridae family.
Article Abstract
Phage O4 of Pseudomonas aeruginosa was previously visualized as a short-tailed virus using a transmission electron microscope. In this work, the O4 genome was characterized to be a linear dsDNA molecule comprising 50509 bp with 76 predicted genes located in five clusters. Mass spectrometry showed that the O4 virion contains 6 putative structural proteins, 2 putative enzymes, and 7 hypothetical proteins. By analyzing a Tn5G transposon mutation library, eight genes, wbpR, wbpV, wbpO, wbpT, wbpS, wbpL, galU, and wzy, were identified and confirmed responsible for the phage-resistant phenotype; all of them are related to the synthesis of O-specific antigen (OSA) of lipopolysaccharide (LPS), indicating that OSA is the receptor for the adsorption of phage O4. Comparative genomic analysis revealed that the phage O4 genome shares little similarity to any known podovirus, indicating that phage O4 is classifiable as a novel member of the Podoviridae family.
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| http://dx.doi.org/10.1007/s00705-018-3866-y | DOI Listing |
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