It is established that local X-ray irradiation of the rabbit hind limb produces a decrease in Ca2+, Mg2+-ATP-dependent formation of electric potentials difference on the membrane of the sarcoplasmic reticulum (SR). These results agree with the observed decrease in the Ca2+-ATPase activity of SR membranes and increase in their electric conduction after irradiation.
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Int J Mol Sci
December 2024
Department of Pharmacy and Biotechnology, University of Bologna, Via Irnerio n.42, 40126 Bologna, Italy.
Disaccharide trehalose has been proven in many cases to be particularly effective in preserving the functional and structural integrity of biological macromolecules. In this work, we studied its effect on the electron transfer reactions that occur in the chromatophores of the photosynthetic bacterium . In the presence of a high concentration of trehalose, following the activation of the photochemistry by flashes of light, a slowdown of the electrogenic reactions related to the activity of the photosynthetic reaction center and cytochtome (cyt) complexes is observable.
View Article and Find Full Text PDFNat Commun
December 2024
Department of Synthetic Biology and Immunology, National Institute of Chemistry, Ljubljana, Slovenia.
Inflammasomes are defense complexes that utilize cytokines and immunogenic cell death (ICD) to stimulate the immune system against pathogens. Inspired by their dual action, we present cytokine-armed pyroptosis as a strategy for boosting immune response against diverse types of tumors. To induce pyroptosis, we utilize designed tightly regulated gasdermin D variants comprising different pore-forming capabilities and diverse modes of activation, representing a toolbox of ICD inducers.
View Article and Find Full Text PDFACS Appl Mater Interfaces
January 2025
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
A biofuel cell is an electrochemical device using exoelectrogen or biocatalysts to transfer electrons from redox reactions to the electrodes. While wild-type microbes and natural enzymes are often employed as exoelectrogen and biocatalysts, genetically engineered or modified organisms have been developed to enhance exoelectrogen activity. Here, we demonstrated a redox-enzyme integrated microbial fuel cell (REI-MFC) design based on an exoelectrogen-enhancing strategy that reinforces the electrogenic activity of MR1 by displaying an extra redox enzyme on the cell surface.
View Article and Find Full Text PDFEnviron Sci Technol
December 2024
CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei 230026, China.
Uranium is the primary fuel for nuclear energy, critical for sustainable, carbon-neutral energy transitions. However, limited terrestrial resources and environmental risks from uranium contamination require innovative immobilization and recovery solutions. In this work, we present a novel uranium recovery method using programmable electroactive living materials (ELMs).
View Article and Find Full Text PDFMicrosyst Nanoeng
December 2024
Department of Electrical & Computer Engineering, Bioelectronics & Microsystems Laboratory, State University of New York at Binghamton, Binghamton, NY, 13902, USA.
We introduce a groundbreaking proof-of-concept for a novel glucose monitoring transducing mechanism, marking the first demonstration of a spore-forming microbial whole-cell sensing platform. The approach uses selective and sensitive germination of Bacillus subtilis spores in response to glucose in potassium-rich bodily fluids such as sweat. As the rate of germination and the number of metabolically active germinating cells are directly proportional to glucose concentration, the electrogenic activity of these cells-manifested as electricity-serves as a self-powered transducing signal for glucose detection.
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