[Damage of deltamethrin on tissue and cell DNA in hepatopancreas of Pagrosomus major].

In order to study the toxic effect of deltamethrin on the hepatopancreas of Pagrosomus major with sub-acute exposure, Pagrosomus major were divided into 5 groups with different doses of semi-static exposure. The damage of the hepatopancreas tissue of Pagrosomus major was analyzed by microscopy observation and the DNA damage of the cells was analyzed by comet assay technique after 25 days exposure. The results showed that different degrees of congestion, nuclear enrichment and cell necrosis appeared in hepatopancreas of 0.025, 0.125, 0.250, 0.375 Μg·L exposure concentrations. The higher the exposure concentration, the variations of tissue and cell were more significant. DNA damages in the hepatopancreas cells of all exposed group were observed. The co-met rate, percentage of tail DNA and the olive moment were significantly different from the control group (P<0.05). Linear regression analysis showed that the exposure concentration was significantly positively correlated with the tailing rate and the tail length (P<0.01). The indexes were significantly related to the concentration of deltamethrin exposure with the coefficient of determination between 0.909-0.996. It was suggested that deltamethrin could cause various degree of damage to the tissue and cellular DNAs in the hepatopancreas of Pagrosomus major in a linear responsive manner.