Introduction: () is an important opportunistic pathogen causes serious community and hospital-acquired infections, which is highly resistant to antibiotics. We aimed to determine the frequency of multidrug resistant (MDR) and molecular typing of clinical isolates of .
Methodology: One hundred isolates of were collected from clinical samples in three general hospitals in Kermanshah. The antimicrobial susceptibility and extended-spectrum beta-lactamases (ESBL) production of isolates were determined using disk diffusion and combined disk methods, respectively. The gene, class I and II integrons were detected using polymerase chain reaction. The positive isolates were selected for genotyping using pulsed-field gel electrophoresis (PFGE).
Results: MDR phenotype was observed in 56% of isolates. The 40% of isolates were ESBL positive and 35 isolates contained . Class I and II of integrons were detected in 50 (89.2%) and 39 (69.6%) of MDR isolates, respectively. PFGE patterns of positive isolates indicated 19 clusters (X) with different genotype patterns.
Conclusions: The study findings highlight the concern of circulating MDR strains of with and class I and II integrons in Kermanshah hospitals. The presence of integrons among isolates may facilitate the spread of new resistance genes in this bacterium. Therefore, surveillance for the spread of MDR strains of this bacterium is recommended in hospitals.
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| http://dx.doi.org/10.4103/ijabmr.IJABMR_333_16 | DOI Listing |
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