Objective: To establish a T7 promoter based reverse genetics system competent for the rescue of bovine parainfluenza virus type 3 (BPIV3).
Methods: We constructed three helper plasmids of px8δT-PT1-bPIV3-NP, px8δT-PT1-bPIV3-P and px8δT-PT1-bPIV3-L and one minigenome plasmid of pSC11-bPIV3-EGFP containing open reading frame (ORF) of the enhanced green fluorescent protein (EGFP) and cis-acting elements including BPIV3 leader region, gene start (GS), gene end (GE) and trailer region. All these plasmids are under the control of T7 promoter and identified by restriction endonuclease analysis. We rescued the pSC11-bPIV3-EGFP by two different methods. Then, we observed the fluorescence expression over time with fluorescence microscopy.
Results: We successfully constructed a reverse genetic system based 4 plasmids under the control of T7 promoter and finished the rescue operation to the minigenome of BPIV3.
Conclusion: This system can be further applied to investigate the function of BPIV3 genome by deletion and mutation of its genes.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Impact of a trace mineral injection at weaning on growth, behavior, and inflammatory, antioxidant, and immune responses of beef calves.
Transl Anim Sci
December 2024
Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS 79074-460, Brazil.
Two experiments evaluated the effects of an injectable trace mineral (ITM) solution at weaning on trace mineral (TM) status, inflammatory and antioxidant responses, grazing behavior, response to vaccination, and growth of beef calves. Experiment 1 used 86 Nellore calves (40 females and 46 males; body weight [BW] = 198 ± 30.8 kg; 8 ± 1 mo of age) weaned (day 0) and assigned into one of two treatments: saline (0.
View Article and Find Full Text PDFFrequency of Bovine Respiratory Disease Complex Bacterial and Viral Agents Using Multiplex Real-Time qPCR in Quebec, Canada, from 2019 to 2023.
Vet Sci
December 2024
Biovet Inc., Division of Antech Diagnostics and Mars Petcare Science & Diagnostics Company, Saint-Hyacinthe, QC J2S 8W2, Canada.
The bovine respiratory disease complex (BRD) is a multifactorial disease caused by various bacterial and viral pathogens. Using rapid pathogen detection techniques is helpful for tailoring therapeutic and preventive strategies in affected animals and herds. The objective of this study was to report the frequency of 10 pathogens by multiplex RT-qPCR on samples submitted for BRD diagnosis to a diagnostic laboratory (Biovet Inc.
View Article and Find Full Text PDFEstablishment of a Real-Time Reverse Transcription Recombinase-Aided Isothermal Amplification (qRT-RAA) Assay for the Rapid Detection of Bovine Respiratory Syncytial Virus.
Vet Sci
November 2024
Hebei Key Laboratory of Preventive Veterinary Medicine, Hebei Normal University of Science & Technology, Qinhuangdao 066600, China.
Background: Bovine respiratory syncytial virus (BRSV) is a significant cause of bovine respiratory disease, resulting in significant losses to the cattle industry. For rapid detection of BRSV, a real-time recombinase-aided isothermal amplification assay (qRT-RAA) based on the gene of BRSV was developed in this study.
Results: The developed qRT-RAA assay showed good exponential amplification of the target fragment in 20 min at a constant temperature of 39 °C.
Mycoplasma ovipneumoniae identified as the main aetiological agent of respiratory disease in goats from a case-control study in Savannakhet province of Lao PDR.
Vet Microbiol
December 2024
School of Environmental and Rural Science, University of New England, Armidale, NSW, Australia.
Clinical signs of respiratory disease are common in Lao goats. To identify the causative agents involved in this clinical syndrome, a matched case-control study was conducted across 70 smallholder goat holdings in Savannakhet province. Fifty paired nasal swab samples were collected from goats with respiratory signs (cases) and unaffected (control) goats from 27 goat holdings.
View Article and Find Full Text PDFRinderpest and peste des petits ruminants: state of play in disease eradication efforts.
Rev Sci Tech
December 2024
Rinderpest virus and peste des petits ruminants (PPR) virus are highly pathogenic viruses causing disease primarily in cattle and small ruminants, respectively. Although the post-eradication process for rinderpest has been largely successful, gaps in preparedness for a future rinderpest reappearance remain, and the virus is still held in some facilities that have not been registered or inspected, posing a threat to the global community. The PPR Global Eradication Programme will need to overcome significant hurdles to reach a world free of the disease by 2030.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!