Phenolic acid content, antioxidant and cytotoxic activities of four species.

Phenolic acid composition, antioxidant, and cytotoxic activities in leaves of four (Crassulaceae) species were evaluated. Determination of phenolic acid contents were conducted by an optimized LC-ESI-MS/MS method. The results show that Raym.-Hamet & H. Perrier (using ASE extraction) and (Lam.) Pers. contain the highest amounts of phenolic acids, while Engl. the lowest ones. Among phenolic acids ferulic, caffeic and protocatechuic acids were occurring in the highest quantities in the analysed species. The greatest amounts of ferulic and protocatechuic acids were found in and Moreover, the antiradical and cytotoxic activities of extracts were investigated. All tested extracts possessed antioxidant activity. The obtained IC values (μg/mL) ranged from 49.9 μg/mL to 1410 μg/mL, indicating a large variation of the activity of the analysed extracts. Cytotoxicity assays revealed dose-dependent effects in the cells lines tested. Only extract showed a high cytotoxicity against the H-9 human T cell line. Other extracts (, , ) showed more pronounced cytotoxicity towards J45.01 cells (human acute lymphoblastic leukaemia T cells). The present study demonstrated that extracts have significant antioxidant and cytotoxic effects. This suggests that these species can be used as new sources of natural antioxidants and potential anticancer compounds.