[Preparation of I Labeled Collagen-Chitosan Microspheres and It's Antitumor Effect on Human Liver Cancer].

Objective: To prepare iodine-131(I) labeled biodegradable microspheres with chitosan and collagen for treating liver cancer.

Methods: Collagen-chitosan microspheres (CCMSs) were prepared with type-Ⅰ collagen and chitosan using emulsification-chemical cross-linking method. The size of the CCMSs were determined by electron microscope. I-CCMSs were achieved using Chloramine-T. The labelling rate of I was recorded. The stability of I-CCMSs were evaluated in PBS or human blood serum through 192 h incubation. The HepG2 model was established in nude mice 28 d after subcutaneous injection of 10 HepG2 cells. The model mice were sacrificed 7 d after injection of I-CCMSs,blank microspheres,or PBS (five mice in each group) into the HepG2 tumor xenografts. Samples of various organs were collected to determine the distribution of I-CCMSs. The curative effect of I-CCMSs on liver cancer was assessed by staining with HE for histological analyses.

Results: CCMSs were synthesized with a smooth and spherical shape and an average diameter of (5.1±1.2) μm. A radiolabeling rate of 86.10% was achieved. I radio-loading remained stable: 92.00% in saline and 83.00% in human serum after 192 h incubation. I was mainly concentrated in the subcutaneous tumor tissues. Potent curative effects of I-CCMSs on subcutaneous tumor tissues were demonstrated.

Conclusion: Biodegradable CCMSs were successfully prepared and radiolabeled. The I-CCMSs exhibited potential curative effects on liver cancer,with high stability and