The pathogen of Cytospora sp. was considered as the target fungus to evaluate the bio-control potential of antagonistic actinomycetes against Cytospora sp. in the present study, which was isolated from the rhizosphere soil of apple tree. Moreover, the antagonistic effect of actinomycetes against Cytospora sp. growth was determined by the method of confrontation and growth rate, and the screened antagonistic strain was identified by the method of morphology and molecular biology. Also, the inhibitory activity of strain ZZ-9 against the conidium germination and mycelia growth of Cytospora sp. was determined in vivo, and its bio-control effect on Cytospora sp. growth was determined in detached apple twigs. The results showed that fifteen strains of actinomycetes had the inhibitory effect on Cytospora sp. growth, among all the isolated strains, which accounted for 18.8% and especially the inhibitory rate of eight strains was more than 50%. In addition, among all the screened strains, the strain ZZ-9 presented the highest inhibitory rate of 96.4%, which was significantly higher than those of the other isolated strains. The strain ZZ-9 was initially identified as Streptomyces rochei according to cultural characteristics, physiological and biochemical properties and 16S rDNA analysis. The nucleotide sequences in GenBank accession number was registered as KT986228. Different dilution of ZZ-9 fermentation had significant inhibitory effect on Cytospora sp. conidium germination and mycelia growth. The inhibitory rates of the 50 times fermentation on both conidium germination and mycelia growth were more than 80%. The inhibited mycelia's color was deepened, the mycelia branches were increased, and the ends of hyphae were swelled and deformed, even the protoplasm was concentrated and released. The bio-control effect of the ZZ-9 stock solution on Cytospora sp. growth was more than 75% in detached apple twigs. Thus, our results indicated that the strain ZZ-9 could be used for controlling apple tree Valsa canker in vivo and vitro.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.13287/j.1001-9332.201610.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification and growth-promoting effect of Paecilomyces lilacinus a biocontrol fungi for walnut rot disease.
PLoS One
December 2024
Tarim University/Key Laboratory of Integrated Pest Management (IPM) of Xinjiang Production and Construction Corps in Southern Xinjiang, College of Agriculture, Alar, China.
Cytospora chrysosperma is the primary pathogen responsible for walnut rot disease, affecting a wide variety of hosts. Currently, chemical agents, particularly agricultural Fungicides, are commonly utilized for the prevention and management of walnut rot. However, this practice has led to the development of drug-resistant pathogens, complicating disease control efforts.
View Article and Find Full Text PDFTrehalose biosynthetic genes are involved in the development and pathogenesis in the poplar canker fungus .
Phytopathology
November 2024
Beijing Forestry University, College of Forestry, Beijing Forestry University, No.35, Tsinghua Eastern Road, Haidian District, Beijing, Beijing, China, 100083;
Poplar Cytospora canker, caused by , is one of the most destructive and widespread poplar diseases worldwide, especially in northern China. However, our current understanding of its pathogenic mechanisms remains limited. Here, we showed that trehalose biosynthetic genes, such as trehalose-6-phosphate synthase 1 (Tps1), trehalose-6-phosphate phosphatase (Tps2), and the regulatory subunit (Tps3), play important roles in the development and virulence of .
View Article and Find Full Text PDFEffects of LDS17 on growth, rhizosphere soil enzyme activities, and microflora, and genome-wide analysis of plant growth-promoting genes.
Microbiol Spectr
May 2024
Department of Life Sciences, Changzhi University, Changzhi, China.
Unlabelled: is a perennial herbaceous liana with medicinal value. It is critical to promote growth through effective and sustainable methods, and the use of plant growth-promoting bacteria (PGPB) is a promising candidate. In this study, we isolated a PGPB, LDS17, that produced a highly active 1-aminocyclopropane-1-carboxylate deaminase from the rhizosphere.
View Article and Find Full Text PDFUncovering the antifungal activities of wild apple-associated bacteria against two canker-causing fungi, Cytospora mali and C. parasitica.
Sci Rep
March 2024
Laboratory of Molecular and Biochemical Genetics, Institute of Genetics and Plants Experimental Biology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan.
Cytospora canker has become a devastating disease of apple species worldwide, and in severe cases, it may cause dieback of entire trees. The aim of this study was to characterize the diversity of cultivable bacteria from the wild apple microbiota and to determine their antifungal ability against the canker-causing pathogenic fungi Cytospora mali and C. parasitica.
View Article and Find Full Text PDFThe mitogen-activated protein kinase module CcSte11-CcSte7-CcPmk1 regulates pathogenicity via the transcription factor CcSte12 in Cytospora chrysosperma.
Stress Biol
January 2024
State Key Laboratory of Efficient Production of Forest Resources, Beijing, China.
The pathogen Cytospora chrysosperma is the causal agent of poplar canker disease and causes considerable economic losses in China. Mitogen-activated protein kinase (MAPK) cascades play a crucial role in mediating cellular responses and Pmk1-MAPKs are indispensable for pathogenic related processes in plant pathogenic fungi. In previous studies, we demonstrated that the CcPmk1 acts as a core regulator of fungal pathogenicity by modulating a small number of master downstream targets, such as CcSte12.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!