Total internal reflection microscopy is mainly used in its fluorescence mode and is the reference technique to image fluorescent proteins in the vicinity of cell membranes. Here, we show that this technique can easily become a phase microscope by simply detecting the coherent signal resulting from the interference between the field scattered by the probed sample and the total internal reflection. Moreover, combining several illumination angles permits generating synthetic aperture reconstructions with improved resolutions compared to standard label-free microscopy techniques.

Download full-text PDF

Source
http://dx.doi.org/10.1364/OL.43.002173DOI Listing

Publication Analysis

Top Keywords

total internal
12
internal reflection
12
synthetic aperture
8
reflection microscopy
8
phase imaging
4
imaging synthetic
4
aperture super-resolution
4
super-resolution total
4
microscopy total
4
microscopy fluorescence
4

Similar Publications

The purpose of this study was to evaluate the psychometric properties of the Chinese version of the Revised Indebtedness Scale (IS-R-C) in mainland China. A total of 1057 university students participated in this study using a two-wave whole-group sampling method. Sample 1, consisting of 537 participants, was used for item analysis and exploratory factor analysis (EFA) of the Revised Indebtedness Scale (IS-R).

View Article and Find Full Text PDF

Psychometric properties of the English and Hindi versions of the Brief Inventory of Thriving for use among Indian adolescents.

Sci Rep

December 2024

Faculty of Education, Centre for Wellbeing Science, The University of Melbourne, Level 2, 100 Leicester Street, Carlton, VIC, 3010, Australia.

The Brief Inventory of Thriving (BIT) provides a holistic measure of well-being, but has only been validated for adults, and does not have a Hindi version. The present study investigated the unidimensional structure, internal consistency, convergent/discriminant, and criterion validity of both the original English version of the BIT (BIT-E) and its Hindi-translated version (BIT-H) among adolescents in India. Further, we tested measurement invariance across these two language versions, gender, and academic disciplines.

View Article and Find Full Text PDF

Immune checkpoint inhibitor (ICI) treatment has proven successful for advanced melanoma, but is associated with potentially severe toxicity and high costs. Accurate biomarkers for response are lacking. The present work is the first to investigate the value of deep learning on CT imaging of metastatic lesions for predicting ICI treatment outcomes in advanced melanoma.

View Article and Find Full Text PDF

Introduction: End-stage kidney disease patients face a critical decision regarding kidney replacement therapy options, which include kidney transplantation, hemodialysis, or peritoneal dialysis (PD). This study aims to evaluate the impact of nurse-led education (NE) alone vs. NE combined with peer support on the patients' decision over PD treatment in chronic kidney disease patients.

View Article and Find Full Text PDF

Background: Trichophyton indotineae, formerly described as T. mentagrophytes rDNA-ITS genotype VIII, has recently been identified as a novel species within the T. mentagrophytes complex.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!