AI Article Synopsis
- A study explored major protein antigens recognized by antibodies in infected mice, focusing on identifying proteins that provoke immune responses.
- Through immunoblotting and proteomic analysis, researchers identified three key antigens: pyruvate phosphate dikinase, Hsp-85, and β-tubulin, with β-tubulin being the most significant.
- Immunizing mice with recombinant β-tubulin enhanced their immune response and provided protection against infection, suggesting this antigen could be a candidate for Chagas disease vaccines.
Article Abstract
Few studies investigate the major protein antigens targeted by the antibody diversity of infected mice with . To detect global IgG antibody specificities, sera from infected mice were immunoblotted against whole extracts. By proteomic analysis, we were able to identify the most immunogenic proteins. We identified three major antigens as pyruvate phosphate dikinase, Hsp-85, and β-tubulin. The major protein band recognized by host IgG was β-tubulin. The β-tubulin gene was cloned, expressed in , and recombinant β-tubulin was obtained. Infection increased IgG reactivity against recombinant β-tubulin. A single immunization of mice with recombinant β-tubulin increased specific IgG reactivity and induced protection against infection. These results indicate that repertoire analysis is a valid approach to identify antigens for vaccines against Chagas disease.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5909033 | PMC |
| http://dx.doi.org/10.3389/fimmu.2018.00671 | DOI Listing |
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