AiV-1 is considered an emerging human enteric pathogens and foodborne transmission has been documented as an important source of exposure for humans, chiefly in relation to non-safe, risky food habits. We surveyed the presence of AiV-1 in retail shellfish, including oysters and mussles, identifying the virus in 3/170 (1.8%) of the analysed samples. The AiV-1 positive samples were of different geographic origin. Upon sequence analysis of a portion of the 3CD junction region, two AiV strains identified from harvesting areas in Northern Italy were characterised as genotype B and displayed 99-100% identity at the nucleotide level to other AiV-1 strains detected in sewages in Central Italy in 2012, suggesting that such strains are stably circulating in Italian ecosystems. Interestingly, a strain identified from mussles harvested in Southern Italy could not be characterised firmly, as inferred in the Bayesian analysis and by sequence comparison, indicating that different AiV strains are also circulating in Italy. Viral contamination in retail shellfish challenges the microbiological guidelines for food control and requires the development and optimization of additional diagnostic and prevention strategies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fm.2018.02.013 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Contamination of Japanese Retail Foods With Enterotoxigenic Clostridium Perfringens Spores.
J Food Prot
January 2025
Kawasaki City Institute for Public Health, 3-25-13 Tonomachi, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-0821, Japan.
The contamination of Japanese retail foods and the intestinal contents of animals with the spores of enterotoxigenic Clostridium perfringens were investigated by analyzing clostridial toxin genes (cpa and cpe) using a culture method and PCR. Enterotoxigenic C. perfringens was detected in 12.
View Article and Find Full Text PDFPrevalence of Cronobacter spp. in tropical seafood from Mumbai, India: Comparative study of isolation media and PCR detection.
J AOAC Int
November 2024
Fish Processing Technology Department, ICAR-Central Institute of Fisheries Education, Versova, Mumbai-400061, India.
Article Synopsis
- Cronobacter species are opportunistic pathogens found in various foods, making their isolation from seafood, like fish and shellfish, challenging due to their coexistence with similar bacteria in aquatic environments.
- This study analyzed 75 seafood samples from retail markets, revealing a 32% incidence rate of Cronobacter, with dried fish showing the highest prevalence (48%).
- The best method for isolating Cronobacter spp. involved using specific enrichment broths and isolation media, emphasizing dried fish's role as a significant reservoir for the pathogen, particularly C. sakazakii.
Microbial profiling of oysters from a processing plant and retail products: Analysis based on culture-dependent methods and 16S rRNA gene sequencing.
Food Res Int
November 2024
Department of Food Science and Biotechnology, Ewha Womans University, Seoul, South Korea. Electronic address:
Oysters (Crassostrea gigas) are one of the most consumed shellfish globally. However, there is a lack of comprehensive microbiome studies that include the processing and distribution stages of oysters. The present study used both culture-based methods and 16S rRNA sequencing to produce comprehensive microbial profiles of oysters in two parts: (1) an oyster processing plant that processes raw and frozen oysters (n = 57) and (2) retail oyster products across two seasons (winter and spring) (n = 112).
View Article and Find Full Text PDFHigh prevalence of multidrug-resistant Escherichia coli in retail aquatic products in China and the first report of mcr-1-positive extended-spectrum β-lactamase-producing E. coli ST2705 and ST10 in fish.
Int J Food Microbiol
October 2023
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, Guangdong Province 510070, China.
Article Synopsis
- Contamination of retail aquatic products in China by multidrug-resistant (MDR) E. coli poses a significant health risk to consumers, with 40% of samples found to contain E. coli and 19.9% showing resistance to multiple drugs.
- The study analyzed 849 samples from fish, shrimp, and shellfish across 39 cities and revealed high levels of antibiotic resistance, especially to tetracycline and trimethoprim-sulfamethoxazole.
- A variety of antibiotic resistance genes were identified, with a concerning prevalence of resistant strains, including colistin-resistant isolates associated with the mcr-1 gene, highlighting poor hygiene practices in retail markets.
Hazard Characterization of Antibiotic-resistant Aeromonas spp. Isolated from Mussel and Oyster Shellstock Available for Retail Purchase in Canada.
J Food Prot
November 2024
Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, A.L. 2204E, Ottawa, Ontario K1A 0K9, Canada. Electronic address:
Article Synopsis
- The study investigates the presence of antimicrobial-resistant (AMR) bacteria in live mussels and oysters, emphasizing the risks these foods pose to human health.
- Out of 180 samples tested, 52% contained third-generation cephalosporin-resistant bacteria, mainly from Serratia, Aeromonas, and Rahnella species.
- Further genomic analysis of selected Aeromonas strains revealed multiple antibiotic resistance genes and mobile genetic elements, underscoring the complexities of assessing foodborne bacterial risks through genomic tools.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!