Bacilysin is one of the simplest antimicrobial peptides and has drawn great attention for its excellent performance against Candida albicans. In this study, the antifungal mechanism of bacilysin was investigated. The target enzyme glucosamine-6-phosphate synthase (GFA) was expressed heterologously in Escherichia coli and its inhibition by bacilysin and derivatives was studied. It was concluded that bacilysin could be hydrolyzed by a proteinase of C. albicans, and that the released product, anticapsin, then inhibited the aminotransferase activity of GFA. This result was verified by molecular simulation, and the interaction mode of anticapsin with GFA was detailed, which provides data for the development of novel antifungal drugs. Transport of bacilysin into fungal cells was also simulated and it was shown that bacilysin is more readily transported into cells than anticapsin. Thus, our findings support a mechanism whereby bacilysin is transported into fungal pathogens, hydrolyzed to anticapsin, which then inhibits GFA.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00894-018-3645-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multifaceted growth promotion and biocontrol of Agroathelia rolfsii and induction of defense mechanism by Bacillus amyloliquefaciens SS-CR10 on chilli.
Physiol Plant
November 2024
Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysuru, Karnataka, India.
Plant-growth-promoting (PGP) endophytic bacteria are beneficial microorganisms that can help plants withstand biotic stress caused by fungal phytopathogens. In the present study, 78 endophytic bacterial isolates were isolated from chilli (Capsicum annuum L.).
View Article and Find Full Text PDFUnravelling the secondary metabolome and biocontrol potential of the recently described species Bacillus nakamurai.
Microbiol Res
November 2024
Microbial Processes and Interactions (MiPI), Teaching and Research Centre (TERRA), Gembloux Agro-BioTech, University of Liège, Avenue de la Faculté 2B, Gembloux 5030, Belgium. Electronic address:
In the prospect of novel potential biocontrol agents, a new strain BDI-IS1 belonging to the recently described Bacillus nakamurai was selected for its strong in vitro antimicrobial activities against a range of bacterial and fungal phytopathogens. Genome mining coupled with metabolomics revealed that BDI-IS1 produces multiple non-ribosomal secondary metabolites including surfactin, iturin A, bacillaene, bacillibactin and bacilysin, together with some some ribosomally-synthesized and post-translationally modified peptides (RiPPs) such as plantazolicin, and potentially amylocyclicin, bacinapeptin and LCI. Reverse genetics further showed the specific involvement of some of these compounds in the antagonistic activity of the strain.
View Article and Find Full Text PDFComplete Genome Sequence Analysis of MC4-2 Strain That against Tobacco Black Shank Disease.
Int J Genomics
March 2024
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China.
The MC4-2 bacterium strain was isolated and purified from the intestine as a biocontrol agent with good antagonistic effect against the pathogens of a soil-borne disease called tobacco black shank. The MC4-2 strain was found to have good broad-spectrum inhibition by plate stand-off test. Based on 16S rRNA and genes, ANI analysis, and other comparative genomics methods, it was determined that the MC4-2 strain was .
View Article and Find Full Text PDFPerception of the Biocontrol Potential and Palmitic Acid Biosynthesis Pathway of H2 through Merging Genome Mining with Chemical Analysis.
J Agric Food Chem
March 2024
Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang 550025, China.
has been widely studied for its potential to protect plants from pathogens. Here, we report the whole genome sequence of H2, which was isolated from the tea garden soil of Guiyang Forest Park. Strain H2 showed a broad spectrum of antagonistic activities against many plant fungal pathogens and bacteria pathogens, including the rice blast fungus , and showed a good field control effect against rice blast.
View Article and Find Full Text PDFWhole genome analysis of 160, biological control agent of corn head smut.
Microbiol Spectr
April 2024
Universidad Politécnica de Pachuca, Zempoala, Hidalgo, Mexico.
Article Synopsis
- Corn head smut, a corn disease caused by a fungus, has been a persistent issue in central Mexico's Mezquital Valley, where strain 160 was found to reduce disease and improve crop yields.
- * The study sequenced the genome of strain 160, revealing it has a size of 4,297,348 bp with significant genetic components for producing various antimicrobials, including three clusters for surfactin.
- * This research enhances the understanding of strain 160’s potential for biological control, contributing to advancements in biofungicides and strategies for increasing agricultural productivity.*
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!