Lanthanum chloride-induced conformational changes of bovine liver catalase: A computational and biophysical study.

Int J Biol Macromol

Post Graduate Department of Biotechnology, Utkal University, Bhubaneswar 751014, Odisha, India; Department of Biochemistry, North-Eastern Hill University, Shillong 792022, Meghalaya, India. Electronic address:

Published: August 2018

AI Article Synopsis

  • Researchers studied how lanthanum chloride (LaCl) affects the activity and structure of bovine liver catalase (BLC) in various buffer solutions at 25°C.
  • They found that higher concentrations of LaCl decreased catalase activity, with the order of effectiveness being HEPES > MOPS > Tris > Phosphate buffer.
  • The study showed that LaCl induces structural changes in BLC, leading to reduced activity, with the most significant changes occurring in HEPES and MOPS buffers, while phosphate buffer's reduced activity was linked to changes in its buffering capacity.

Article Abstract

We have investigated the effects of lanthanum chloride (LaCl) on catalytic activity and conformation of bovine liver catalase (BLC) in different buffer solutions in vitro at 25 °C. Higher concentration of the salt caused decrease in catalase activity in the following order, HEPES > MOPS > Tris > Phosphate buffer. Results obtained from circular dichroism, fluorescence and absorption spectroscopy and from computational docking studies indicate that reduction in activity of BLC by LaCl is due to induction of conformational changes. Lanthanum-induced reduced BLC activity in MOPS, HEPES and Tris buffer is characterized by a significant loss in native fluorescence and increase in absorbance spectra of BLC. Nevertheless, the change in secondary conformation of BLC was maximum in HEPES and MOPS followed by Tris and least in Phosphate buffer. Therefore, the significant loss of BLC activity in phosphate buffer at higher molar concentration of lanthanum is attributed to the change in buffering capacity of the buffer. The conformational transition of BLC by LaCl was followed as a function of concentration. Therefore, the reduced BLC activity is directly controlled by lanthanum-induced conformational change of BLC in HEPES, MOPS and Tris buffer and indirectly controlled by the change in buffering capacity of the phosphate buffer.

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http://dx.doi.org/10.1016/j.ijbiomac.2018.04.116DOI Listing

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