A pot experiment with N tracing techniques was designed to study the effect of potassium application on nitrogen transfer, photosynthetic characteristics and nitrogen metabolism enzyme activities in two different growth stages of sweet potato. Results indicated that potassium application significantly increased the shoot N distribution rate. Compared with control, the N transfer rate of K treatment increased by 76.2% and the total accumulation of N increased by 92.1% in tuber formation period. Different with tuber formation period, shoot N distribution rate decreased from 33.7% to 24.4%, but the root N distribution rate increased from 5.8% to 17% with the increase of K application in tuber rapid growth stage. Especially, root N accumulation of K treatment was 3 times of the CK. During the two growth stages, nitrate reductase, glutamate dehydrogenase, glutamine synthetase, glutamate synthase and net photosynthetic rate all increased with the increase of K application. Stepwise regression analysis showed that nitrogen metabolism enzyme activities (nitrate reductase, glutamate dehydrogenase, glutamine synthetase, glutamate synthase) and P were the main factors to affect the N transfer and distribution of sweet potato (R=0.965,R=0.942). Path analysis showed that nitrate reductase and glutamate dehydrogenase activities were the key factors to influence N distribution to the shoot in tuber formation period, while glutamate dehydrogenase, glutamate synthase activities were the key factors to influence N distribution to the tuber in tuber rapid growth stage.
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http://dx.doi.org/10.13287/j.1001-9332.201611.021 | DOI Listing |
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National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
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School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
Nitrogen (N) is one of the three major elements required for plant growth and development. It is of great significance to study the effects of different nitrogen application levels on the growth and root exudates of Phlomoides rotata, and can provide a theoretical basis for its scientific application of fertilizer to increase production. In this study, Phlomoides rotata were grown under different nitrogen conditions for two months.
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Department of Veterinary Medicine and Epidemiology, UC Davis School of Veterinary Medicine,
Bearded dragons () are a common reptile species kept under human care and suffer from a wide range of diseases for which plasma biochemistry is used as a first-line diagnostic test. There is limited information available regarding tissue enzyme activities and origin that could assist in interpreting the bearded dragon plasma biochemistry enzymology profile. The aim of this study was to characterize the tissue activities of seven enzymes routinely used in the reptile biochemistry panel: alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), glutamate dehydrogenase (GLDH), lactate dehydrogenase (LDH), and creatine kinase (CK) in 12 adult inland bearded dragons in 13 tissues, plasma, and red blood cells.
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