There are numerous reports on embryo culture media and conditions in the laboratory, as the subject is multifaceted and complex, reflecting the variation in practice. In this scoping review, we attempt to approach the topic of culture media and conditions from the practitioners' perspective aiming to highlight, in a comprehensive fashion, important aspects regarding the options available, introduce points of debate and controversy, while maintaining the viewpoint of the practicing embryologist's concerns.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6000787 | PMC |
http://dx.doi.org/10.21873/invivo.11261 | DOI Listing |
Nicotinamide adenine dinucleotide (NAD(H)) and its metabolites function as crucial regulators of physiological processes, allowing cells to adapt to environmental changes such as nutritional deficiencies, genotoxic factors, disruptions in circadian rhythms, infections, inflammation, and exogenous substances. Here, we investigated whether elevated NAD(H) levels in oocytes enhance their quality and improve developmental competence following in vitro fertilization (IVF). Bovine cumulus-oocyte complexes (COCs) were matured in a culture medium supplemented with 0-100 μM nicotinamide mononucleotide (NMN), a precursor of NAD(H).
View Article and Find Full Text PDFJ Oral Biosci
December 2024
Department of Maxillofacial Orthognathics, Division of Maxillofacial and Neck Reconstruction, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, 1-5-45, Bunkyo-ku, Tokyo, 113-8549, Japan.
Objectives: To investigate the effects of hypoxia on tooth germ development in mice and explore the underlying mechanisms.
Methods: Tooth germs were extracted from E14.5 mouse embryos and divided into the control and hypoxia groups for organ culture.
J Virol
December 2024
Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.
Protein kinase R (PKR) is an interferon-induced antiviral protein activated by autophosphorylation in response to double strand DNA (dsRNA) and other stimuli. Activated PKR causes translation inhibition and apoptosis, and it contributes to proinflammatory responses, cell growth, and differentiation. Mouse adenovirus type 1 (MAV-1) counteracts PKR by causing its degradation via a viral protein, early region 4 open reading frame 6 (E4orf6).
View Article and Find Full Text PDFZhongguo Shi Yan Xue Ye Xue Za Zhi
December 2024
Life Science School of Hebei University, Baoding 071000, Hebei Province, China.
J Assist Reprod Genet
December 2024
Reproductive Medical Center, People's Liberation Army Joint Logistic Support Force 924Th Hospital, Guilin, Guangxi, People's Republic of China.
Purpose: Selection of optimal embryo transfer strategies for IVF patients treated with antagonist protocols.
Methods: A retrospective study was conducted to assess whether whole embryo culture to the blastocyst stage could enhance the cumulative live birth rate (CLBR). The study included data from the first oocyte retrieval cycle of 4131 patients who underwent IVF treatment between January 2018 and June 2022.
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