The B19 parvovirus is a cause of bone marrow failure in humans. B19 is toxic to erythroid progenitor cells in vitro. Viral products possibly responsible for toxicity were explored by transfection of cloned B19 genome into HeLa cells. The nonstructural (NS) protein was detected in cells 30 h after transfection. Plasmids containing the B19 genome were transfected with selectable marker genes in stable transformation assays. Plasmids that contained the left side of the B19 genome, which encodes the NS protein of the virus, inhibited antibiotic-resistant colony formation. Transformation occurred when NS protein expression was blocked by mutation. Suppression of transformation by NS protein was not tissue specific, suggesting a role for NS protein in toxicity for nonpermissive cells without parvovirus replication or virion accumulation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC253725 | PMC |
| http://dx.doi.org/10.1128/JVI.62.8.2884-2889.1988 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Human parvovirus B19 as an unusual cause of Guillain-Barré syndrome: A case report.
IDCases
December 2024
Laboratoire de Virologie, CNR des Entérovirus et Parechovirus, CHU de Clermont-Ferrand, France.
Human Parvovirus B19 (B19V) is rarely observed in patients with Guillain-Barré syndrome. We report the case of a patient with rapidly progressive functional impotence of the limbs. B19V was detected in both blood and CSF samples.
View Article and Find Full Text PDFVirological characterization of Parvovirus B19 isolated during the atypical 2023-2024 outbreak in France.
J Infect
January 2025
Microbiology department, Necker-Enfants malades Hospital, AP-HP, Université Paris Cité, Paris, France; Université Paris Cité, URP 7328 FETUS, Paris, France.
Background: A Parvovirus B19 (B19V) outbreak has been reported in Europe in 2023-2024. The aims of this study were 1) to describe the incidence of primary cases from 2012 to 2024 in one French hospital 2) to analyze the genome of 2023 strains 3) to identify virological profiles according to the clinical presentations of B19V infection.
Methods: The incidence of B19V primary cases was studied through an interrupted time-series analysis.
GATA1 transcription factor targets the gene expression of B19 virus in HEK293 cell line.
Ann Med Surg (Lond)
December 2024
Department of Laboratory Hematology and Blood Banking, Dezful University of Medical Sciences, Iran.
Background/aim: B19 virus (B19V) is a single-strand DNA virus that has specific tropism to erythroid progenitor cells (EPCs). The virus enters the cells via P antigen and coreceptors and induces infection and cell apoptosis. GATA1 has a high expression in EPC and is a critical transcription factor for the cells development and differentiation.
View Article and Find Full Text PDFArticle Synopsis
- - Human parvovirus B19 typically causes mild illness but can pose serious risks to pregnant individuals, those with weakened immune systems, and people with chronic blood disorders; recent B19 activity has surged in several European countries.
- - In the U.S., no standard B19 surveillance exists, but studies found a significant rise in IgM antibodies, indicating recent infections, showing a notable increase from less than 1.5% (2020-2023) to 9.9% in Q2 of 2024.
- - A similar spike was observed in donor plasma testing, with 20% positive for B19 nucleic acid amplification testing in June 2024, up from under 2% in previous years
Intra-host genomic diversity and integration landscape of human tissue-resident DNA virome.
Nucleic Acids Res
November 2024
Department of Virology, University of Helsinki and Helsinki University Hospital, Haartmaninkatu 3, P.O. Box 21, FI-00014, Helsinki, Finland.
The viral intra-host genetic diversities and interactions with the human genome during decades of persistence remain poorly characterized. In this study, we analyzed the variability and integration sites of persisting viruses in nine organs from thirteen individuals who died suddenly from non-viral causes. The viruses studied included parvovirus B19, six herpesviruses, Merkel cell (MCPyV) and JC polyomaviruses, totaling 127 genomes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!