AI Article Synopsis
- SERS tags are gaining popularity for their high sensitivity and ability to analyze multiple samples simultaneously.
- A major limitation has been the difficulty in selecting suitable Raman dyes to attach to metal nanoparticles.
- This study presents a new method that allows for the easy preparation of Raman nanoprobes, broadening the types of dyes that can be used, and demonstrating their effectiveness in multiplexed immunohistochemistry.
Article Abstract
Surface-enhanced Raman scattering (SERS) tags have attracted tremendous attention in diverse fields owing to their outstanding sensitivity and multiplexing capability. However, the selection of Raman dyes that can be immobilized onto metal nanoparticles is very limited, because certain chemical groups are needed in the dye molecules to interact either with the metal surface or through some intermediate layers. Here, we report a simple, rapid, and robust platform methodology for the one-pot preparation of Raman nanoprobes without the constraints of Raman dye chemical structures. We demonstrate this general approach by immobilizing dye molecules on silver nanoparticle surfaces that were difficult to incorporate previously, and show their applications in multiplexed immunohistochemistry (IHC). We expect that this platform nanotechnology will significantly expand the library of SERS tags and their biomedical uses.
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| http://dx.doi.org/10.1039/c8nr00564h | DOI Listing |
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