Aim: Telomere length (TL) measurement by quantitative polymerase chain reaction (PCR) has been widely accepted, but limited information regarding its validation with a gold-standard technique is available.
Materials & Methods: We measured TL by Southern blot and monochrome multiplex quantitative PCR (MMqPCR) and validated the results of TL in leukocytes of 94 participants with mean age 43.2 years, BMI 19-41 (mean 27.8 ± 4.3) kg/m.
Results: A significant positive correlation was observed between TL measured by MMqPCR and Southern blot assay (correlation coefficient r = +0.896, p < 0.0001). The inter- and intra-assay CVs of the MMqPCR assay were 5.3 and 4.07%, respectively.
Conclusion: We observed that experimental discrepancies have an impact on TL analysis and there is a need to improve the optimum conditions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5905642 | PMC |
| http://dx.doi.org/10.4155/fsoa-2017-0115 | DOI Listing |
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