Posttransformational vector amplification (PTVA) is widely used to enrich the gene-copy number in Pichia pastoris. We engineered two test strains for PTVA studies and demonstrate that the PTVA process results in the amplification of a fragment with the resistant gene flanked by two homologous arms instead of the entire vector.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mimet.2018.04.013 | DOI Listing |
Publication Analysis
Top Keywords
posttransformational vector
8
vector amplification
8
amplification ptva
8
pichia pastoris
8
insight method
4
method posttransformational
4
ptva
4
ptva pichia
4
pastoris posttransformational
4
ptva enrich
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!