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| http://dx.doi.org/10.3324/haematol.2017.180844 | DOI Listing |
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Upregulation of nuclear protein Hemgn by transcriptional repressor Gfi1 through repressing PU.1 contributes to the anti-apoptotic activity of Gfi1.
J Biol Chem
November 2024
Department of Biological Sciences, University of Toledo, Toledo, Ohio, USA. Electronic address:
Article Synopsis
- - Gfi1 is a crucial transcriptional repressor involved in blood cell development, mainly working through its SNAG domain to recruit the histone demethylase LSD1, affecting gene regulation.
- - The study reveals Gfi1's ability to enhance the expression of the Hemgn gene via a specific promoter region, with interactions from transcription factors like Ikaros (which activates Hemgn) and PU.1 (which represses it).
- - Gfi1's upregulation of Hemgn not only occurs through repression of PU.1 but also contributes to Gfi1's protective role against cell death caused by stress, independent of the p53 protein.
Neutrophil metalloproteinase driven spleen damage hampers infection control of trypanosomiasis.
Nat Commun
September 2023
Laboratory for Biomedical Research, Department of Environmental Technology, Food Technology and Molecular Biotechnology KR01, Ghent University Global Campus, Incheon, South Korea.
Recent blood transcriptomic analysis of rhodesiense sleeping sickness patients has revealed that neutrophil signature genes and activation markers constitute the top indicators of trypanosomiasis-associated inflammation. Here, we show that Trypanosoma brucei infection results in expansion and differentiation of four splenic neutrophil subpopulations, including Mki67Birc5Gfi1Cebpe proliferation-competent precursors, two intermediate immature subpopulations and CebpbSpi1Irf7Mcl1Csf3r inflammation reprogrammed mature neutrophils. Transcriptomic scRNA-seq profiling identified the largest immature subpopulation by Mmp8/9 positive tertiary granule markers.
View Article and Find Full Text PDFThe scaffolding function of LSD1/KDM1A reinforces a negative feedback loop to repress stem cell gene expression during primitive hematopoiesis.
iScience
January 2023
Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, 2000 Circle of Hope Drive, Salt Lake City, UT 84112, USA.
Lsd1/Kdm1a functions both as a histone demethylase enzyme and as a scaffold for assembling chromatin modifier and transcription factor complexes to regulate gene expression. The relative contributions of Lsd1's demethylase and scaffolding functions during embryogenesis are not known. Here, we analyze two independent zebrafish mutant lines and show Lsd1 is required to repress primitive hematopoietic stem cell gene expression.
View Article and Find Full Text PDFHMG20B stabilizes association of LSD1 with GFI1 on chromatin to confer transcription repression and leukemia cell differentiation block.
Oncogene
October 2022
Leukaemia Biology Laboratory, Cancer Research UK Manchester Institute, The University of Manchester, Manchester Cancer Research Centre Building, 555 Wilmslow Road, Manchester, M20 4GJ, UK.
Pharmacologic inhibition of LSD1 induces molecular and morphologic differentiation of blast cells in acute myeloid leukemia (AML) patients harboring MLL gene translocations. In addition to its demethylase activity, LSD1 has a critical scaffolding function at genomic sites occupied by the SNAG domain transcription repressor GFI1. Importantly, inhibitors block both enzymatic and scaffolding activities, in the latter case by disrupting the protein:protein interaction of GFI1 with LSD1.
View Article and Find Full Text PDFGFI1 Cooperates with IKZF1/IKAROS to Activate Gene Expression in T-cell Acute Lymphoblastic Leukemia.
Mol Cancer Res
April 2022
Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah.
Unlabelled: Growth factor independence-1 (GFI1) is a transcriptional repressor and master regulator of normal and malignant hematopoiesis. Repression by GFI1 is attributable to recruitment of LSD1-containing protein complexes via its SNAG domain. However, the full complement of GFI1 partners in transcriptional control is not known.
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