Transfectable functional genomics libraries are traditionally the workhorses of functional genomics screening using reporter gene assays. These libraries offer insight into fundamental cellular processes governing health and disease and can be utilized in an arrayed fashion which makes them uniquely suited to deconvolute complicated disease phenotypes and dissect biological networks that would otherwise be inaccessible. Here we give an overview of the principles for the generation, screening and data analysis of such arrayed libraries. Specifically we cover the differences between the various transfectable reagents, library selection and handling, and data analysis to offer a comprehensive understanding of these important technologies and how to apply them.
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