RPA is known to stimulate the helicase activity of Werner syndrome protein (WRN), but the exact stimulation mechanism is not understood. We use single-molecule FRET and magnetic tweezers to investigate the helicase activity of WRN and its stimulation by RPA. We show that WRN alone is a weak helicase which repetitively unwind just a few tens of base pairs, but that binding of multiple RPAs to the enzyme converts WRN into a superhelicase that unidirectionally unwinds double-stranded DNA more than 1 kb. Our study provides a good case in which the activity and biological functions of the enzyme may be fundamentally altered by the binding of cofactors.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961295 | PMC |
| http://dx.doi.org/10.1093/nar/gky272 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genomic selection in forest trees comes to life: unraveling its potential in an advanced four-generation population.
Front Plant Sci
October 2024
Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Recursos Biológicos, Centro de Investigación en Recursos Naturales, Buenos Aires, Argentina.
Genomic Selection (GS) in tree breeding optimizes genetic gains by leveraging genomic data to enable early selection of seedlings without phenotypic data reducing breeding cycle and increasing selection intensity. Traditional assessments of the potential of GS in forest trees have typically focused on model performance using cross-validation within the same generation but evaluating effectively realized predictive ability (RPA) across generations is crucial. This study estimated RPAs for volume growth (VOL), wood density (WD), and pulp yield (PY) across four generations breeding of .
View Article and Find Full Text PDFCircadian rhythms of melatonin and its relationship with anhedonia in patients with mood disorders: a cross-sectional study.
BMC Psychiatry
February 2024
The School of Mental Health and Psychological Sciences, Anhui Medical University, 230032, Hefei, China.
Background: Mood disorders are strongly associated with melatonin disturbances. However, it is unclear whether there is a difference in melatonin concentrations and melatonin circadian rhythm profiles between depression and bipolar disorder. In addition, the relationship between anhedonia, a common symptom of affective disorders, and its melatonin circadian rhythm remains under-investigated.
View Article and Find Full Text PDFTime to Flip the Approach: Retroperitoneoscopic Adrenalectomy.
J Surg Res
April 2024
Department of General Surgery, Royal Brisbane and Women's Hospital, Herston, Queensland, Australia.
Introduction: Retroperitoneoscopic adrenalectomy (RPA) has gained increasing popularity with its excellent perioperative outcomes and direct surgical access compared to other adrenalectomy approaches. We review perioperative outcomes of RPA by a specialized endocrine surgeon before and after expert intensive trainings (EITs), and to that of other laparoscopic adrenalectomy approaches at our center over a 9-year period, aiming to ascertain if RPA is worth the steep learning curve.
Material And Methods: One hundred twenty one adrenalectomies were performed between January 2014 to June 2022.
Replication Protein A, the Main Eukaryotic Single-Stranded DNA Binding Protein, a Focal Point in Cellular DNA Metabolism.
Int J Mol Sci
January 2024
Centre for Chromosome Biology, School of Biological and Chemical Sciences, Biochemistry, University of Galway, H91 TK33 Galway, Ireland.
Replication protein A (RPA) is a heterotrimeric protein complex and the main single-stranded DNA (ssDNA)-binding protein in eukaryotes. RPA has key functions in most of the DNA-associated metabolic pathways and DNA damage signalling. Its high affinity for ssDNA helps to stabilise ssDNA structures and protect the DNA sequence from nuclease attacks.
View Article and Find Full Text PDFAssociation between ambient particulate matters and anhedonia among patients with depression.
Environ Sci Pollut Res Int
January 2024
The School of Mental Health and Psychological Sciences, Anhui Medical University, Hefei, 230032, China.
Recent studies have linked ambient air pollution to depression. Anhedonia is a core symptom of depression which severely impacts on prognosis. The present study aims to investigate the association of PM2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!