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| http://dx.doi.org/10.3201/eid2405.172076 | DOI Listing |
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Molecular Detection of Tick-Borne Bacterial Pathogens in Patients With Undifferentiated Febrile Illness in India Using a Flow Chip Assay.
Cureus
December 2024
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, New Delhi, IND.
Background: Tick-borne diseases (TBDs) play a crucial role in human morbidity and mortality, as ticks are highly effective in spreading diseases by transmitting harmful pathogens to humans and animals. The last few decades have seen an increase in the number of recognized tick-borne pathogens and the incidence of TBD worldwide. Several of these diseases are ubiquitous in India.
View Article and Find Full Text PDFDevelopment of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock.
Front Vet Sci
December 2024
Centre for Tropical Livestock Genetics and Health (CTLGH), Roslin Institute, University of Edinburgh, Edinburgh, United Kingdom.
One of the principal limitations on livestock productivity in sub-Saharan Africa is the constraining effect of infectious diseases, including tick-borne blood pathogens. Currently, diagnostic markers for these pathogens are species or genus specific, making it challenging to implement high-throughput screening methods. The aim of this study was to develop and validate a novel high-throughput diagnostic tool capable of detecting a range of important haemopathogens in livestock.
View Article and Find Full Text PDFIdentification of ticks and tick-borne pathogens of wildlife necropsy cases submitted to the SANBI National Zoological Gardens, South Africa.
Vet Parasitol Reg Stud Reports
October 2024
South African National Biodiversity Institute, P.O. Box 754, Pretoria 0001, South Africa; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Ondestepoort, Pretoria 0110, South Africa.
Ticks are arachnid blood-feeding parasites, which infest livestock, wildlife, and humans, transmitting medically and veterinary significant pathogens. Their biodiversity and distribution in wild animals remains complex. This study analysed archived tick samples (n = 48) from the South African Biodiversity Institute (SANBI) Wildlife Biobank utilizing morphology and genetic analyses of the 16S rRNA and COI (DNA barcoding) mitochondrial genes to identify ticks collected among 13 vertebratesavian, reptilian, and mammalian host species.
View Article and Find Full Text PDFDetection of tick-borne pathogens in the pangolin tick, Amblyomma javanense, from Vietnam and Laos, including a novel species of Trypanosoma.
Acta Trop
December 2024
Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary; HUN-REN-UVMB Climate Change, New Blood-sucking Parasites and Vector-borne Pathogens Research Group, Hungary. Electronic address:
Two species of Southeast Asian pangolins (the Chinese pangolin, Manis pentadactyla and the Malayan or Sunda pangolin, Manis javanica) are critically endangered species. Therefore, knowledge on their parasitic infections is very important, especially considering ticks that can transmit which pathogens. In this study, 32 pangolin ticks (Amblyomma javanense), that were collected in Vietnam and Laos, were analyzed with molecular methods for the presence of tick-borne pathogens.
View Article and Find Full Text PDFDevelopment and evaluation of a TaqMan® real-time PCR assay for species-specific detection of Ehrlichia canis.
Diagn Microbiol Infect Dis
December 2024
Instituto de Investigación de la Cadena Láctea (IdICaL) CONICET - INTA; Ruta 34 km 227 - CP: 2300 - Rafaela, Santa Fe, Argentina. Electronic address:
The aim of this work was to develop a real-time PCR assay with a TaqMan® probe that detects a species-specific part of the 16S rDNA gene of Ehrlichia canis. Canine blood samples (n = 207), collected and tested by a conventional PCR assay within a study conducted by De Salvo et al., were simultaneously analyzed with the novel designed real-time PCR, and the results of both assays were compared.
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