Paenibacillus yanchengensis sp. nov., isolated from farmland soil.

A Gram-variable, short-rod-shaped, motile, spore-forming, strictly aerobic and alkaliresistant bacterium, designed strain J-3, was isolated from farmland soil sampled in Yancheng city, Jiangsu province, China. Optimal growth occurred at 30 °C, pH 7.0-8.0 and 0.5 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain J-3 was most closely related to Paenibacillusripae HZ1 (96.8 %), followed by Paenibacillussputi KIT00200-70066-1 (94.7 %). The major cellular fatty acids were anteiso-C15 : 0 and C16 : 0. The dominant respiratory quinone was menaquinone-7 and the DNA G+C content was 41.2 mol%. The major polar lipids of strain J-3 were aminolipid, phospholipid, diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid and phosphatidylethanolamine. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. On the basis of genotypic and phenotypic data, strain J-3 represents a novel species of the genus Paenibacillus, for which the name Paenibacillus yanchengensis sp. nov. is proposed. The type strain is J-3 (=KCTC 33926=CGMCC 1.16455).