Antioxidant and hepato-preventive effect of extract against carbon tetrachloride-induced hepatotoxicity in rats and characterisation of its bioactive compounds by HPLC-MS.

The objective of this study is to explore the preventive effects of ethyl acetate fraction from leaf extract (EACA), associated with its phytochemical content, against the toxic impacts of acute exposure to carbon tetrachloride (CCl) in the liver of adult rats. HPLC analysis of ethyl acetate fraction from extract revealed eight compounds. Administration of a single dose of CCl caused hepatoxicity as monitored by an increase in lipid peroxidation (thiobarbituric acid reactive substances) and in protein carbonyl level but a decrease in antioxidant markers in the liver tissue. The pre-treatment with EACA; significantly prevented the increased plasma levels of hepatic markers and lipid levels induced by CCl in rats. Furthermore, this fraction ameliorated biochemical and histological parameters as compared to CCl-treated group. Our results suggest that contains promising substances to counteract the CCl intoxication and can be efficient in the prevention of hepatotoxicity complications.