AI Article Synopsis
- Subsurface cistern (SSC) is a specialized endoplasmic reticulum compartment located near the plasma membrane in neurons, likely involved in regulating intracellular calcium levels.
- High potassium and NMDA stimulation in hippocampal neurons resulted in a rapid decrease in SSC-PM contact areas, indicating a structural decoupling under excitatory conditions.
- This reduction in contact area may function as a protective mechanism for neurons to prevent calcium overload during increased neuronal activity.
Article Abstract
Subsurface cistern (SSC) in neuronal soma and primary dendrites is a specialized compartment of endoplasmic reticulum (ER) that is in close apposition (10 nm) with the plasma membrane (PM). ER-PM contact areas are thought to be involved in intracellular calcium regulation. Here, structural changes of SSC in hippocampal neurons were examined by electron microscopy upon depolarization with high K (90 mM) or application of NMDA (50 μM) in rat dissociated cultures as well as organotypic slice cultures. The number and average length of SSC-PM contact areas in neuronal somas significantly decreased within 30 s under excitatory condition. This decrease in SSC-PM contact area progressed with time and was reversible. These results demonstrate a structural decoupling between the SSC and the PM upon stimulation, suggesting that there may be a functional decoupling of the calcium regulation. Because SSC-PM contact areas may mediate calcium influx, the decrease in contact area may protect neurons from calcium overload upon heightened stimulation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5902880 | PMC |
| http://dx.doi.org/10.1186/s13041-018-0366-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prevalence of Chronic Hand Eczema in adults: A cross-sectional survey of over 60,000 respondents in the general population in Canada, France, Germany, Italy, Spain, and the United Kingdom.
Br J Dermatol
January 2025
Department of Occupational and Environmental Diseases, University Hospital of Centre of Paris, Hotel-Dieu Hospital, and Department of Dermatology, University Hospital of Centre of Paris, Cochin Hospital, AP-HP, Paris, France AP-HP, Paris, France.
Background: The lack of attention to Chronic Hand Eczema (CHE) and the lack of a specific International Classification of Diseases code for CHE may have limited the assessment of CHE prevalence. To date, prevalence estimates have primarily been derived from (partly small) single-country studies.
Objectives: To estimate the annual prevalence of self-reported physician-diagnosed CHE across socio-demographic characteristics among adults in Canada, France, Germany, Italy, Spain, and the United Kingdom (UK).
Morphology of the Soft Palate Tonsil Mucosa of Collared Peccary (Pecari tajacu, Linnaeus, 1758).
Anat Histol Embryol
January 2025
Laboratório de Morfologia e Atividade Física, São Paulo State University, Rio Claro, São Paulo, Brazil.
Collared Peccary (Pecari tajacu, Linnaeus, 1758) is a mammalian Tayassuidae species from tropical to semi-arid areas. The morphological features of the oral cavity in this species were identified and described. Tonsils are secondary lymphoid organs essential for contact with antigens due to food and air intake.
View Article and Find Full Text PDFDesign and Characterization of Novel Polymeric Hydrogels with Protein Carriers for Biomedical Use.
Int J Mol Sci
December 2024
Department of Materials Engineering, Faculty of Materials Engineering and Physics, Cracow University of Technology, 37 Jana Pawla II Av., 31-864 Krakow, Poland.
Hydrogels are three-dimensional polymeric matrices capable of absorbing significant amounts of water or biological fluids, making them promising candidates for biomedical applications such as drug delivery and wound healing. In this study, novel hydrogels were synthesized using a photopolymerization method and modified with cisplatin-loaded protein carriers, as well as natural extracts of nettle () and chamomile ( L.).
View Article and Find Full Text PDFDirect Knitting of Pincer Palladium Complexes into Hyper-crosslinked Polymers for Superior Catalytic Performance in Suzuki-Miyaura Reactions.
Chem Asian J
January 2025
Huazhong University of Science and Technology, School of Chemisry & Chemical Engineering, 1037 Luoyu Road, 430074, Wuhan, CHINA.
Using a direct knitting strategy, we successfully prepared a novel heterogeneous catalyst consisting of pyridine-bridged bis(imidazolium-2-ylidene) palladium complexes (CNC-Pd) embedded in a knitted network polymer. The resulting catalysts (HCP-CNC-Pd-d) exhibited high specific surface areas of 982 m2 g-1 with microporous and mesoporous structures. The large surface area enhances contact between the substrate and the catalytic center, while the strong chelation between CNC and the metal ion ensures the catalyst's durability.
View Article and Find Full Text PDFPopulation genomics and connectivity of Vazella pourtalesii sponge grounds of the northwest Atlantic with conservation implications of deep sea vulnerable marine ecosystems.
Sci Rep
January 2025
Department of Biological Sciences, University of Bergen, Bergen, Norway.
Sponges are key ecosystem engineers that shape, structure and enhance the biodiversity of marine benthic communities globally. Sponge aggregations and reefs are recognized as vulnerable marine ecosystems (or VMEs) due to their susceptibility to damage from bottom-contact fishing gears. Ensuring their long-term sustainability, preservation, and ecosystem functions requires the implementation of sound scientific conservation tools.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!