Surface plasmon resonance (SPR) has become a well-recognized label-free technique for measuring the binding kinetics between biomolecules since the invention of the first SPR-based immunosensor in 1980s. The most popular and traditional format for SPR analysis is to monitor the real-time optical signals when a solution containing ligand molecules is flowing over a sensor substrate functionalized with purified receptor molecules. In recent years, rapid development of several kinds of SPR imaging techniques have allowed for mapping the dynamic distribution of local mass density within single living cells with high spatial and temporal resolutions and reliable sensitivity. Such capability immediately enabled one to investigate the interaction between important biomolecules and intact cells in a label-free, quantitative, and single cell manner, leading to an exciting new trend of cell-based SPR bioanalysis. In this Trend Article, we first describe the principle and technical features of two types of SPR imaging techniques based on prism and objective, respectively. Then we survey the intact cell-based applications in both fundamental cell biology and drug discovery. We conclude the article with comments and perspectives on the future developments. Graphical abstract Recent developments in surface plasmon resonance (SPR) imaging techniques allow for label-free mapping the mass-distribution within single living cells, leading to great expansions in biomolecular interactions studies from homogeneous substrates functionalized with purified biomolecules to heterogeneous substrates containing individual living cells.
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http://dx.doi.org/10.1007/s00216-018-1008-8 | DOI Listing |
Adv Sci (Weinh)
January 2025
SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, 16419, South Korea.
Molecular diagnosis limitations, including complex treatment processes, low cost-effectiveness, and operator-dependent low reproducibility, interrupt the timely prevention of disease spread and the development of medical devices for home and outdoor uses. A newly fabricated gold nanopillar array-based film is presented for superior photothermal energy conversion. Magnifying the metal film surface-to-volume ratio increases the photothermal energy conversion efficiency, resulting in a swift reduction in the gene amplification reaction time.
View Article and Find Full Text PDFPhys Chem Chem Phys
January 2025
Department of Chemical Engineering, Ataturk University, 25240 Erzurum, Turkey.
The combination of plasmonic metals and MXene, as a new and interesting member of the 2D material class, may provide unique advantages in terms of low cost, versatility, flexibility, and improved activity as an ideal surface-enhanced Raman spectroscopy (SERS) platform. Despite the recent progress, the present studies on the utilization of plasmonic metal/MXene-based SERS systems are quite limited and thereby benefits of the extraordinary properties of this combination cannot be realized. In this study, for the first time, we propose layer-by-layer (LbL) thin films of TiC MXene and gold nanoparticles (AuNPs) as a robust SERS platform (TiC/AuNPs).
View Article and Find Full Text PDFSoft Matter
January 2025
Faculty of Chemistry, Ho Chi Minh City University of Science, Vietnam National University, Ho Chi Minh City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City 70000, Vietnam.
Surface-enhanced Raman spectroscopy (SERS) is a highly sensitive analytical technique with excellent molecular specificity. However, separate pristine nanoparticles produce relatively weak Raman signals. It is necessary to focus on increasing the "hot-spot" density generated at the nanogaps between the adjacent nanoparticles (second-generation SERS hotspot), thus significantly boosting the Raman signal by creating an electromagnetic field.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Dept of Biochemistry & Center for Biophysics and Quantitative Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA.
Bio-Layer Interferometry (BLI) is a technique that uses optical biosensing to analyze interactions between molecules. The analysis of molecular interactions is measured in real-time and does not require fluorescent tags. BLI uses disposable biosensors that come in a variety of formats to bind different ligands including biotin, hexahistidine, GST, and the Fc portion of antibodies.
View Article and Find Full Text PDFJ Appl Microbiol
January 2025
School of Basic Medicine, Jiamusi University, Jiamusi 154000, China.
Aims: The aim of this study was to identify sesamin as a Casein hydrolase P (ClpP) inhibitor and to determine whether it could attenuate the virulence of methicillin-resistant Staphylococcus aureus (MRSA).
Methods And Results: Through fluorescence resonance energy transfer (FRET) screening, a natural compound sesamin demonstrated a significant inhibitory effect on ClpP enzyme activity with an IC50 of 20.62 μg/mL.
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