In Vitro Study on the Plasma-Polymerization of Propionaldehyde and Allylglycidyl Ether for Immobilization of Recombinant Human Bone Morphogenetic Protein-2.

In this study, recombinant human bone morphogenetic protein-2 (rhBMP-2) was directly immobilized on the plasma-polymerized propionaldehyde (PA) and allylglycidyl ether (AGE) surface through the imine bonding and epoxy-amine bonding, respectively. Aldehyde and epoxide plasma-polymerization were carried out at plasma power 60 W for 10 min and monomers were used to PA and AGE. After the plasma-polymerization and rhBMP-2 immobilization, substrate surfaces were characterized by contact angle, field emission scanning electron microscopy, and attenuated total reflectance Fourier transform infrared. In addition, the biological activities of MC3T3-E1 cells were evaluated by initial adhesion and alkaline phosphate (ALP) activity. The rhBMP-2 immobilized PA and AGE surfaces promoted significantly higher ALP activity of MC3T3-E1 cells than pristine surface.