The purpose of this study was to examine the direct toxicity of PM2.5 collected from Beijing on human umbilical vein endothelial cells (HUVEC). A Cell Counting Kit 8 (CCK8) assay demonstrated that PM2.5 exposure decreased the proliferation of HUVECs in a dose-dependent manner. We also found that PM2.5 exposure induced autophagy in HUVECs, as evidenced by: (1) an increased number of double-membrane vesicles; (2) enhanced conversion and punctuation of the microtubule-associated protein light chain 3 (LC3); and (3) decreased levels of the selective autophagy substrate p62 in a time-dependent manner. Furthermore, promoting autophagy in PM2.5-exposed HUVECs with rapamycin increased the cell survival rate, whereas inhibiting autophagy via 3-methyladenine significantly decreased cell survival. These results demonstrate that PM2.5 exposure can induce cytotoxicity and autophagy in HUVECs and that autophagy play a protective role against PM2.5-induced cytotoxicity. The findings of the present study imply a direct toxic effect of PM2.5 on HUVECs and provide novel insight into the mechanism of cardiovascular diseases caused by PM2.5 exposure.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jes.2017.05.019 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Spatiotemporal Patterns and Equity Analysis of Premature Mortality Due to Ischemic Heart Disease Attributable to PM Exposure in China: 2007-2022.
Toxics
August 2024
School of Geological Engineering and Geomatics, Chang'an University, Xi'an 710054, China.
Long-term exposure to PM pollution increases the risk of cardiovascular diseases, particularly ischemic heart disease (IHD). Current assessments of the health effects related to PM exposure are limited by sparse ground monitoring stations and applicable disease research cohorts, making accurate health effect evaluations challenging. Using satellite-observed aerosol optical depth (AOD) data and the XGBoost-PM25 model, we obtained 1 km scale PM exposure levels across China.
View Article and Find Full Text PDFMaternal Exposure to Environmental Air Pollution and Premature Rupture of Membranes: Evidence from Southern China.
Med Sci Monit
May 2024
Department of Maternal Health Care, Maternal and Child Health Hospital of Tongling, Tongling, Anhui, China (mainland).
BACKGROUND Exposure to air pollution (AP) during pregnancy is associated with pre-labor rupture of membranes (PROM). However, there is limited research on this topic, and the sensitive exposure windows remain unclear. The present study assessed the association between AP exposure and the risk of PROM, as well as seeking to identify the sensitive time windows.
View Article and Find Full Text PDFAmbient PM and specific sources increase inflammatory cytokine responses to stimulators and reduce sensitivity to inhibitors.
Environ Res
July 2024
Department of Environmental Medicine and Climate Science, Icahn School of Medicine at Mt. Sinai, New York, NY, USA; Institute for Climate Change, Environmental Health, and Exposomics, Icahn School of Medicine at Mt. Sinai, New York, NY, USA.
Article Synopsis
- Ambient exposure to fine particulate matter (PM) is linked to increased health risks, raising the question of how PM sensitizes the immune response in children.
- A study involving 277 children investigated the effects of neighborhood PM on inflammatory responses, revealing that higher PM levels correlate with stronger cytokine reactions, particularly from vehicle emissions and dust.
- Longitudinal findings indicated that residential PM exposure decreased sensitivity to anti-inflammatory agents, but PM levels did not appear to affect biomarkers of low-grade inflammation.
Long-term exposure to ambient air pollution and greenness in relation to pulmonary tuberculosis in China: A nationwide modelling study.
Environ Res
November 2022
School of Public Health, Guangzhou Medical University, Guangzhou, 511436, China. Electronic address:
Previous studies have attempted to clarify the relationship between the occurrence of pulmonary tuberculosis (PTB) and exposure to air pollutants. However, evidence from multi-centres, particularly at the national level, is scarce, and no study has examined the modifying effect of greenness on air pollution-TB associations. In this study, we examined the association between long-term exposure to ambient air pollutants (PM p.
View Article and Find Full Text PDFPhysical activity attenuates negative effects of short-term exposure to ambient air pollution on cognitive function.
Environ Int
February 2022
Division of Sports Science & Physical Education, Tsinghua University, Beijing 100084, China. Electronic address:
Article Synopsis
- Physical activity has beneficial effects on cognitive function while air pollution has negative impacts, and their effects may influence each other.
- The study tracked 90 healthy young adults' exposure to air pollution and their physical activity levels over several sessions, assessing cognitive function through tests and EEG signals.
- Findings indicate that short-term exposure to air pollution negatively affected executive function, but engaging in more physical activity can help counteract this damage, especially in polluted environments.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!