Poly (ADP-ribose) polymerase 1 (PARP1) is a DNA damage sensor that catalyzes the poly (ADP-ribose) (PAR) onto a variety of target proteins, such as histones, DSB repair factors and PARP1 itself under consumption of NAD. Besides, PARP1 can affect a variety of proteins in noncovalent modification manner to carry out specific cellular functions. Here, we established a method to generate non-radiolabeled free PAR by PARG moderately cleaving PAR from autoPARylated PARP1, and utilized dot-blot assay to determine the interaction between free PAR and interested proteins. The methods to generate free PAR and detect the noncovalent interactions between proteins and free PAR are nonradioactive and convenient, which will facilitate the studies to explore the significance of PAR reading in various biological processes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mcp.2018.04.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prognostic Implications of Occult Nodal Metastases in Patients with Clinically N0 Primary Parotid Gland Cancer.
Medicina (Kaunas)
November 2024
Department of Otolaryngology-Head and Neck Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
The role of occult nodal metastases in patients with parotid gland cancers remains unclear; such metastases are histologically diverse and exhibit unpredictable clinical courses. Here, we evaluated the prognostic utilities of such metastases, including metastases in the intraparenchymal lymph nodes (PARs). We retrospectively reviewed the medical charts of patients who underwent surgery to treat clinically N0 primary parotid gland cancers from 2000 to 2022.
View Article and Find Full Text PDFQuantitative comparison of a mobile, tablet-based eye-tracker and two stationary, video-based eye-trackers.
Behav Res Methods
January 2025
Department Neurophysics, Philipps-Universität Marburg, Fachbereich Physik, AG Neurophysik, Karl-Von-Frisch-Straße 8a, 35043, Marburg, Lahnberge, Germany.
The analysis of eye movements is a noninvasive, reliable and fast method to detect and quantify brain (dys)function. Here, we investigated the performance of two novel eye-trackers-the Thomas Oculus Motus-research mobile (TOM-rm) and the TOM-research stationary (TOM-rs)-and compared them with the performance of a well-established video-based eye-tracker, i.e.
View Article and Find Full Text PDFEvaluation of Machine Learning/Molecular Mechanics End-State Corrections with Mechanical Embedding to Calculate Relative Protein-Ligand Binding Free Energies.
J Chem Theory Comput
January 2025
Exscientia, Schrödinger Building, Oxford Science Park, Oxford OX4 4GE, U.K.
The development of machine-learning (ML) potentials offers significant accuracy improvements compared to molecular mechanics (MM) because of the inclusion of quantum-mechanical effects in molecular interactions. However, ML simulations are several times more computationally demanding than MM simulations, so there is a trade-off between speed and accuracy. One possible compromise are hybrid machine learning/molecular mechanics (ML/MM) approaches with mechanical embedding that treat the intramolecular interactions of the ligand at the ML level and the protein-ligand interactions at the MM level.
View Article and Find Full Text PDFSub-2W tunable laser based on silicon photonics power amplifier.
Light Sci Appl
January 2025
Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Hamburg, Germany.
Article Synopsis
- High-power tunable lasers are highly sought after for their applications in telecom, ranging, and molecular sensing, but integrated photonics has struggled with power due to size limitations.
- The introduction of large-mode-area (LMA) fibers in the late 90s significantly improved the power capability of fiber systems by increasing the optical mode area.
- This study demonstrates a silicon photonics-based LMA power amplifier that achieves an output power of 1.8W over a 60nm tunability range, indicating a potential advancement in integrated lasers for high-power applications that can compete with traditional bench systems.
Power-free plasma separation based on negative magnetophoresis for rapid biochemical analysis.
Microsyst Nanoeng
December 2024
Research Center for Bionic Sensing and Intelligence, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 518055, Shenzhen, China.
We present a versatile platform for label-free magnetic separation of plasma, tailored to accommodate diverse environments. This innovative device utilizes an advanced long-short alternating double Halbach magnetic array, specifically engineered for optimal magnetic separation. The array's adaptability allows for seamless integration with separation channels of varying sizes, enabling static separation of whole blood.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!