ANTIVIRAL ACTIVITY OF Burm.f. LEAVES AGAINST HIV-INFECTED MT-4 CELLS.

Backgrounds: Burm.f. has been known to have anti-HIV activity. This study was conducted to evaluate the effect of incubation time on the antiviral activity of the leaves extract on HIV-infected MT-4 cells . Molecular docking test was also conducted to determine the interaction of alkaloids and flavonoids on the leaves against HIV-1 reverse transcriptase receptor. It is expected that this research will provide scientific information on the development of leaves as an anti-HIV drug.

Materials And Methods: In the activity test, the effect of incubation time on the antiviral activity of leaves on HIV-infected MT-4 cells were evaluated. During the activity test, a parameter of cytolysis effect inhibition on MT-4 cell line was observed after 4 days and 6 days incubation period. The molecular docking test is performed by using Molegro Virtual Docker software to determine the interaction of alkaloid and flavonoid compounds of leaves with HIV-1 reverse transcriptase receptor.

Results: The incubation time influences the CC and EC value. Fractionated-70% ethanol extract of leaves showed a higher anti-HIV activity with EC = 3.045 x 10 μg/mL, SI = 6.309 x 10 (4 days of incubation) and EC = 6.066 μg/mL, SI = 58494.845 (6 days of incubation). From molecular docking test, it was found that flavonoid of leaves could inhibit the activity of HIV reverse transcriptase enzyme.

Conclusion: Fractionated-70% ethanol extract of has potential as an anti-HIV.