Aqueous two-phase systems (ATPSs) were screened for the production of 6-aminopenicillanic acid (6-APA) catalyzed by penicillin acylase, followed by the extractive separation of 6-APA from the reaction mixture. The key point of this study was to find an ATPS exhibiting a large difference in the partition coefficients of the biocatalyst and reaction products. Several ATPSs based on polyethylene glycol (PEG)/phosphate, PEG/citrate, and PEG/dextran were tested. We found that an ATPS consisting of 15 wt% of PEG 4000, 10 wt% of phosphates, 75 wt% of water (pH value 8.0 after dissolution) provided optimal separation of 6-APA from the enzyme. While the 6-APA was mainly found in the top PEG phase, the free enzyme favored the bottom salt-rich phase. This ATPS also fulfils other important requirements: (i) high buffering capacity, reducing an undesirable pH decrease due to the dissociation of phenylacetic acid (the side product of the reaction), (ii) a relatively low cost of the ATPS components, (iii) the possibility of electrophoretic transport of fine droplets as well as the reaction products for both the acceleration of phase separation and the enhancement of 6-APA concentration in the product stream. Extraction experiments in microcapillary and batch systems showed that the transport of 6-APA formed in the salt-rich phase to the corresponding PEG phase could occur within 30 s. The experimental results described form a base of knowledge for the development of continuously operating integrated microfluidic reactors-separators driven by an electric field for the efficient production of 6-APA.
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http://dx.doi.org/10.1016/j.nbt.2018.03.005 | DOI Listing |
J Food Sci
January 2025
College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, China.
Pleurotus ostreatus is a nutrient-dense edible fungus renowned for its delicate texture, appealing flavor, and numerous potential health benefits. Simultaneous extraction within the framework of food resource processing facilitates the concurrent isolation and analysis of multiple target compounds. In this study, an ethanol/salt aqueous two-phase system (ATPS) was employed to extract polysaccharides (PS) and proteins from P.
View Article and Find Full Text PDFCurr Drug Deliv
January 2025
Department of Biopharmaceutical, Lishui University, 1 Xueyuan Road, Lishui, 323000, China.
Background: Overcoming the poor aqueous solubility of small-molecule drugs is a major challenge in developing clinical pharmaceuticals. Felodipine (FLDP), an L-type calcium calcium channel blocker, is a poorly water-soluble drug.
Objectives: The study aimed to explore the potential applications of polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol (Soluplus) stabilized amorphous dispersions for augmenting the oral delivery of poorly water-soluble drugs.
Materials (Basel)
January 2025
Faculty of Technology and Metallurgy, University of Belgrade, 11000 Belgrade, Serbia.
Int J Biol Macromol
January 2025
Guangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology, Guangxi Academy of Agricultural Sciences, Nanning, China; School of Chemistry and Chemical Engineering, Guangxi University, Nanning, China. Electronic address:
In this research, ultrasound-assisted aqueous two-phase extraction (UA-ATPE) was utilized to isolate polysaccharides from Siraitia grosvenorii (SGPs). Based on the results of model optimization experiments, the extraction parameters were determined. The total extraction rate of SGPs was 25.
View Article and Find Full Text PDFMater Horiz
January 2025
Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology (SeoulTech), Seoul 01811, Republic of Korea.
Aqueous two-phase systems (ATPSs) have primarily been developed in the form of emulsions to enhance their utilization in green and biocompatible applications. However, numerous challenges have arisen in forming stable and processable water-in-water (W/W) emulsion systems, as well as in fine-tuning the interconnectivity of their internal structure, which can significantly impact their performance. To effectively address these challenges, we elucidate, for the first time, the root cause of the poor stability of W/W emulsions.
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